MYB52 Negatively Regulates Pectin Demethylesterification in Seed Coat Mucilage

doi:10.1104/pp.17.01771

	MYB52 Negatively Regulates Pectin Demethylesterification in Seed Coat Mucilage
	Shi, Dachuan 1; Ren, Angyan 1; Tang, Xianfeng 2; Qi, Guang 2; Xu, Zongchang 1; Chai, Guohua 2; Hu, Ruibo 2; Zhou, Gongke 2; Kong, Yingzhen 1
刊名	PLANT PHYSIOLOGY
	2018-04-01
卷号	176 期号:4 页码:2737-2749
ISSN号	0032-0889
DOI	10.1104/pp.17.01771
文献子类	Article
英文摘要	Pectin, which is a major component of the plant primary cell walls, is synthesized and methyl-esterified in the Golgi apparatus and then demethylesterified by pectin methylesterases (PMEs) located in the cell wall. The degree of methylesterification affects the functional properties of pectin, and thereby influences plant growth, development and defense. However, little is known about the mechanisms that regulate pectin demethylesterification. Here, we show that in Arabidopsis (Arabidopsis thaliana) seed coat mucilage, the absence of the MYB52 transcription factor is correlated with an increase in PME activity and a decrease in the degree of pectin methylesterification. Decreased methylesterification in the myb52 mutant is also correlated with an increase in the calcium content of the seed mucilage. Chromatin immunoprecipitation analysis and molecular genetic studies suggest that MYB52 transcriptionally activates PECTIN METHYLESTERASE INHIBITOR6 (PMEI6), PMEI14, and SUBTILISIN-LIKE SER PROTEASE1.7 (SBT1.7) by binding to their promoters. PMEI6 and SBT1.7 have previously been shown to be involved in seed coat mucilage demethylesterification. Our characterization of two PMEI14 mutants suggests that PMEI14 has a role in seed coat mucilage demethylesterification, although its activity may be confined to the seed coat in contrast to PMEI6, which functions in the whole seed. Our demonstration that MYB52 negatively regulates pectin demethylesterification in seed coat mucilage, and the identification of components of the molecular network involved, provides new insight into the regulatory mechanism controlling pectin demethylesterification and increases our understanding of the transcriptional regulation network involved in seed coat mucilage formation.
WOS关键词	CELLULOSE SYNTHASE 5 ; PLANT-CELL WALLS ; ARABIDOPSIS-THALIANA ; TRANSCRIPTION FACTORS ; HOMOGALACTURONAN-METHYLTRANSFERASE ; MONOCLONAL-ANTIBODIES ; ADHERENT MUCILAGE ; SECRETORY-CELLS ; EPIDERMAL-CELLS ; LEUNIG-HOMOLOG
WOS研究方向	Plant Sciences
语种	英语
出版者	AMER SOC PLANT BIOLOGISTS
WOS记录号	WOS:000429089100013
资助机构	National Natural Science Foundation of China(31670302 ; National Key Technology RD Program(2015BAD15B03-05) ; Taishan Scholar Program of Shandong ; Elite Youth Program of CAAS ; China Postdoctoral Science Foundation(2015M571173) ; 31470291)
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/10729]
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Kong, Yingzhen
作者单位	1.Chinese Acad Agr Sci, Tobacco Res Inst, Key Lab Tobacco Gene Resources, Qingdao 266101, Shandong, Peoples R China 2.Chinese Acad Sci, Qingdao Engn Res Ctr Biomass Resources & Environm, Shandong Prov Key Lab Energy Genet, Key Lab Biofuels,Qingdao Inst Bioenergy & Bioproc, Qingdao 266101, Peoples R China
推荐引用方式 GB/T 7714	Shi, Dachuan,Ren, Angyan,Tang, Xianfeng,et al. MYB52 Negatively Regulates Pectin Demethylesterification in Seed Coat Mucilage[J]. PLANT PHYSIOLOGY,2018,176(4):2737-2749.
APA	Shi, Dachuan.,Ren, Angyan.,Tang, Xianfeng.,Qi, Guang.,Xu, Zongchang.,...&Kong, Yingzhen.(2018).MYB52 Negatively Regulates Pectin Demethylesterification in Seed Coat Mucilage.PLANT PHYSIOLOGY,176(4),2737-2749.
MLA	Shi, Dachuan,et al."MYB52 Negatively Regulates Pectin Demethylesterification in Seed Coat Mucilage".PLANT PHYSIOLOGY 176.4(2018):2737-2749.