Characterization of 3-ketoacyl-coA synthase in a nervonic acid producing oleaginous microalgae Mychonastes afer

doi:10.1016/j.algal.2018.02.017

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	Characterization of 3-ketoacyl-coA synthase in a nervonic acid producing oleaginous microalgae Mychonastes afer
	Fan, Yong 1; Yuan, Cheng 2; Jin, Yi 1; Hu, Guang-Rong 2; Li, Fu-Li 1
刊名	ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
	2018-04-01
卷号	31 页码:225-231
关键词	Nervonic Acid 3-ketoacyl-coa Synthase Mychonastes Afer Saccharomyces Cerevisiae
ISSN号	2211-9264
DOI	10.1016/j.algal.2018.02.017
文献子类	Article
英文摘要	Nervonic acid (C24:1, Delta 15), a monounsaturated long chain fatty acid, is a major component of mammalian neural tissue. Lack of nervonic acid in human usually leads to neurological disorder. Recently, production of nervonic acid from plant for pharmaceutical, nutraceutical, and functional food received increasing attention. We have previously isolated a microalgal strain Mychonastes afer HSO-3-1, which can accumulate approximately 6% of nervonic acid and <0.5% of erucic acid in triacylglycerol. In this study, the full length of 3-ketoacyl-coA synthase gene MaKCS, encoding the first component of fatty acid elongation complex, was cloned and expressed in Saccharomyces cerevisiae BY4741 under the control of GAL1 promoter. S. cerevisiae harboring pYC230-Gal-MaKCS resulted in the production of 1.5% nervonic acid in total lipid. The expression of KCS gene in M. afer was found to be induced under stress conditions such as nitrogen deficiency and high light, which was revealed by real-time PCR analysis. MaKCS was demonstrated to be able to extend very long chain monounsaturated fatty acids including nervonic acid, which provides insights into how nervonic acid is synthesized in microalgae.
WOS关键词	KETOACYL-COA SYNTHASE ; CHAIN FATTY-ACIDS ; SUBSTRATE-SPECIFICITY ; FAE1 GENE ; FUNCTIONAL-CHARACTERIZATION ; ARABIDOPSIS-THALIANA ; LUNARIA-ANNUA ; ERUCIC-ACID ; RAT-LIVER ; EXPRESSION
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000428687600024
资助机构	Ministry of Science and Technology of China(2014AA022003 ; National Science Foundation of China(U1232126 ; Shandong Province Natural Science Funds for Distinguished Young Scholar(JQ201507) ; 2015BAD15B02) ; 31602154)
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/10719]
专题	青岛生物能源与过程研究所_微生物资源团队
通讯作者	Li, Fu-Li
作者单位	1.Chinese Acad Sci, Qingdao Engn Lab Single Cell Oil, Shandong Prov Key Lab Energy Genet, Key Lab Biofuels, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Synthet Biol, Qingdao 266101, Peoples R China
推荐引用方式 GB/T 7714	Fan, Yong,Yuan, Cheng,Jin, Yi,et al. Characterization of 3-ketoacyl-coA synthase in a nervonic acid producing oleaginous microalgae Mychonastes afer[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2018,31:225-231.
APA	Fan, Yong,Yuan, Cheng,Jin, Yi,Hu, Guang-Rong,&Li, Fu-Li.(2018).Characterization of 3-ketoacyl-coA synthase in a nervonic acid producing oleaginous microalgae Mychonastes afer.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,31,225-231.
MLA	Fan, Yong,et al."Characterization of 3-ketoacyl-coA synthase in a nervonic acid producing oleaginous microalgae Mychonastes afer".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 31(2018):225-231.