Metabolite Profiling of Wheat Seedlings Induced by Chitosan: Revelation of the Enhanced Carbon and Nitrogen Metabolism'

doi:10.3389/fpls.2017.02017

	Metabolite Profiling of Wheat Seedlings Induced by Chitosan: Revelation of the Enhanced Carbon and Nitrogen Metabolism'
	Zhang, Xiaoqian 1,2,3; Li, Kecheng 1,2; Xing, Ronge 1; Liu, Song 1; Li, Pengcheng 1,2
刊名	FRONTIERS IN PLANT SCIENCE
	2017-11-28
卷号	8
关键词	Chitosan Metabolic Profiling Carbon Metabolism Nitrogen Assimilation Wheat Seedlings
ISSN号	1664-462X
DOI	10.3389/fpls.2017.02017
文献子类	Article
英文摘要	Chitosan plays an important role in regulating growth and eliciting defense in many plant species. However, the exact metabolic response of plants to chitosan is still not clear. The present study performed an integrative analysis of metabolite profiles in chitosan-treated wheat seedlings and further investigated the response of enzyme activities and transcript expression related to the primary carbon (C) and nitrogen (N) metabolism. Metabolite profiling revealed that chitosan could induce significant difference of organic acids, sugars and amino acids in leaves of wheat seedlings. A higher accumulation of sucrose content was observed after chitosan treatment, accompanied by an increase in sucrose phosphate synthase (SPS) and fructose 1, 6-2 phosphatase (FBPase) activities as well as an up-regulation of relative expression level. Several metabolites associated with tricarboxylic acid (TCA) cycle, including oxaloacetate and malate, were also improved along with an elevation of phosphoenolpyruvate carboxylase (PEPC) and pyruvate dehydrogenase (PDH) activities. On the other hand, chitosan could also enhance the N reduction and N assimilation. Glutamate, aspartate and some other amino acids were higher in chitosan-treated plants, accompanied by the activation of key enzymes of N reduction and glutamine synthetase/glutamate synthase (GS/GOGAT) cycle. Together, these results suggested a pleiotropic modulation of carbon and nitrogen metabolism in wheat seedlings induced by chitosan and provided a significant insight into the metabolic mechanism of plants in response to chitosan for the first time, and it would give a basic guidance for the future application of chitosan in agriculture.
WOS关键词	PLANT-GROWTH ; BIOLOGICAL-ACTIVITIES ; ARABIDOPSIS ; ASSIMILATION ; EXPRESSION ; ENZYME ; OLIGOSACCHARIDES ; NITRATE ; LEAVES ; CHITOOLIGOSACCHARIDES
WOS研究方向	Plant Sciences
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000416309800001
资助机构	National Natural Science Foundation of China(41406086) ; Key Research and Development Program of Shandong Province(2017GHY15119) ; Nantong Applied Basic Research Projects(MS12015124) ; commonweal item of State Oceanic Administration People's Republic of China(201405038-2) ; Science and Technology Service Network Initiative (STS)(Y72317107L) ; NSFC-Shandong Joint Fund(U1606403) ; Qingdao National Laboratory for Marine Science and Technology(2015ASKJ02)
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/10491]
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Li, Pengcheng
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Xiaoqian,Li, Kecheng,Xing, Ronge,et al. Metabolite Profiling of Wheat Seedlings Induced by Chitosan: Revelation of the Enhanced Carbon and Nitrogen Metabolism'[J]. FRONTIERS IN PLANT SCIENCE,2017,8.
APA	Zhang, Xiaoqian,Li, Kecheng,Xing, Ronge,Liu, Song,&Li, Pengcheng.(2017).Metabolite Profiling of Wheat Seedlings Induced by Chitosan: Revelation of the Enhanced Carbon and Nitrogen Metabolism'.FRONTIERS IN PLANT SCIENCE,8.
MLA	Zhang, Xiaoqian,et al."Metabolite Profiling of Wheat Seedlings Induced by Chitosan: Revelation of the Enhanced Carbon and Nitrogen Metabolism'".FRONTIERS IN PLANT SCIENCE 8(2017).