Effects of Reduced and Enhanced Glycogen Pools on Salt-Induced Sucrose Production in a Sucrose-Secreting Strain of Synechococcus elongatus PCC 7942

doi:10.1128/AEM.02023-17

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 微生物代谢工程团队

	Effects of Reduced and Enhanced Glycogen Pools on Salt-Induced Sucrose Production in a Sucrose-Secreting Strain of Synechococcus elongatus PCC 7942
	Qiao, Cuncun 1,2,5; Duan, Yangkai 1,3; Zhang, Mingyi 1,3,5; Hagemann, Martin 6; Luo, Quan 1,3; Lu, Xuefeng 1,4
刊名	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
	2018
卷号	84 期号:2
关键词	Sucrose Production Glycogen Riboswitch Synechococcus Elongatus Pcc 7942
DOI	10.1128/AEM.02023-17
文献子类	Article
英文摘要	Sucrose and glycogen syntheses in cyanobacteria share the common precursor glucose-1-phosphate. It is generally assumed that lowering glycogen synthesis could drive more carbon toward sucrose synthesis that can be induced by salt stress among cyanobacteria. By using a theophylline-dependent riboswitch system, the expression of glgC, a key gene in glycogen synthesis, was downregulated in a quantitative manner in a sucrose-secreting strain of Synechococcus elongatus PCC 7942. We observed that the stepwise suppression of glycogen synthesis limited rather than stimulated sucrose production in the salt-stressed cells, suggesting that glycogen could serve as a carbon pool for the synthesis of sucrose. Accordingly, we generated glycogen-overproducing strains, but the increased glycogen pool alone did not stimulate sucrose production, indicating that alternative steps limit the carbon flux toward the synthesis of sucrose. Consistent with previous studies that showed that sucrose-phosphate synthase (SPS) catalyzes the rate-limiting step in sucrose synthesis, the combination of glycogen overproduction and sps overexpression resulted in increased sucrose production. Our results indicate that the glycogen and sucrose pools are closely linked in Synechococcus elongatus PCC 7942, and we propose that enhancing the glycogen pool could be a promising strategy for the improvement of sucrose production by cyanobacteria in the presence of a strong sucrose synthesis sink.
WOS关键词	PHOTOSYNTHETIC PRODUCTION ; STRESS RESPONSES ; CYANOBACTERIUM ; MUTANTS ; BIOSYNTHESIS ; CHEMICALS ; TURNOVER ; FUELS
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
语种	英语
WOS记录号	WOS:000419048900015
资助机构	National Science Fund for Distinguished Young Scholars of China(31525002) ; joint Sino-German research project(GZ984) ; National Science Foundation of China(31501003) ; Shandong Taishan Scholarship ; Qingdao Innovative Leading Talent project(15-10-3-15-(31)-zch)
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/10016]
专题	青岛生物能源与过程研究所_微生物代谢工程团队
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Synthet Biol, Qingdao, Peoples R China 3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Qingdao, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Marine Biol & Biotechnol Lab, Qingdao, Peoples R China 5.Univ Chinese Acad Sci, Beijing, Peoples R China 6.Univ Rostock, Inst Biowissensch, Pflanzenphysiol, Rostock, Germany
推荐引用方式 GB/T 7714	Qiao, Cuncun,Duan, Yangkai,Zhang, Mingyi,et al. Effects of Reduced and Enhanced Glycogen Pools on Salt-Induced Sucrose Production in a Sucrose-Secreting Strain of Synechococcus elongatus PCC 7942[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2018,84(2).
APA	Qiao, Cuncun,Duan, Yangkai,Zhang, Mingyi,Hagemann, Martin,Luo, Quan,&Lu, Xuefeng.(2018).Effects of Reduced and Enhanced Glycogen Pools on Salt-Induced Sucrose Production in a Sucrose-Secreting Strain of Synechococcus elongatus PCC 7942.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,84(2).
MLA	Qiao, Cuncun,et al."Effects of Reduced and Enhanced Glycogen Pools on Salt-Induced Sucrose Production in a Sucrose-Secreting Strain of Synechococcus elongatus PCC 7942".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 84.2(2018).