Carboxylate-Selective Chemical Cross-Linkers for Mass Spectrometric Analysis of Protein Structures

doi:10.1021/acs.analchem.7b03789

	Carboxylate-Selective Chemical Cross-Linkers for Mass Spectrometric Analysis of Protein Structures
	Li, Qiang 1,6; Tan, Dan 3; Wang, Jian-Hua 3,4,5; Zhang, Xiaoyun 1,6; Li, Maodong 2; Lei, Xiaoguang 1,6; Dong, Meng-Qiu 3,4,5; Liu, Zhirong 2; Tang, Chun 7; Gong, Zhou 7
刊名	ANALYTICAL CHEMISTRY
	2018-01-16
卷号	90 期号:2 页码:1195-1201
ISSN号	0003-2700
DOI	10.1021/acs.analchem.7b03789
文献子类	Article
英文摘要	Chemical cross-linking coupled with mass spectrometry (CXMS) facilitates structural analysis, of proteins. As current CXMS applications are almost exclusively limitedto lysine residues, they Can only retrieve a small pOrtion of the structural information theoretically accessibleto CXMS. Chemical: cross-linkers targeting the acidic residues Asp/Glu could greatly enhance the power of CXMS. However, it has been difficult to develop chemistries that offer selectivity and efficiency under physiological conditions. Here, we report a class of carboxylate-selective diazo-containing cross-linkers (Diazoker) of which Diazoker 1, with a spacer arm consisting of two ethan1,2-diol units, is the best example. Unlike previously developed carboxylateselective cross-linkers like pimelic acid dihydrazide (PDH), Diazoker 1 does not require a coupling reagent. We tested Diazoker 1 on nine model proteins and found that Diazoker 1 generated an average of 73 cross-linked peptide pairs per protein. Although this is 32% fewer than the number generated by PDH, the Diazoker 1 cross-links have a'higher rate of compatibility with protein crystal structures. From a more complex protein mixture, Diazoker 1 and PDH identified 75 and 76 cross-linked peptide pairs, respectively. The Asp/Glu residues cross-linked by Diazoker 1 are not the same as those cross-linked by PDH. Diazoker 1 favors acidic residues that are less exposed to solvent. In conclusion, Diazoker 1 is complementary to existing cross-linkers and expands the toolkit of CXMS for structural analysis of proteins.
WOS关键词	DISTANCE RESTRAINTS ; ACIDIC RESIDUES ; LINKING ; ASSEMBLIES ; BIOCONJUGATION ; BIOINFORMATICS ; COMPLEXES ; REAGENTS ; BIOLOGY ; PROBES
WOS研究方向	Chemistry
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000423011600024
资助机构	National Key Research and Development Program of China(2017YFA0505200) ; National Key Research and Development Program of China(2017YFA0505200) ; National High Technology Project 973(2014CB849801 ; National High Technology Project 973(2014CB849801 ; NNSFC(21472010 ; NNSFC(21472010 ; 2015CB856200) ; 2015CB856200) ; 21521003 ; 21521003 ; 21561142002 ; 21561142002 ; 21625201) ; 21625201) ; National Key Research and Development Program of China(2017YFA0505200) ; National Key Research and Development Program of China(2017YFA0505200) ; National High Technology Project 973(2014CB849801 ; National High Technology Project 973(2014CB849801 ; NNSFC(21472010 ; NNSFC(21472010 ; 2015CB856200) ; 2015CB856200) ; 21521003 ; 21521003 ; 21561142002 ; 21561142002 ; 21625201) ; 21625201) ; National Key Research and Development Program of China(2017YFA0505200) ; National Key Research and Development Program of China(2017YFA0505200) ; National High Technology Project 973(2014CB849801 ; National High Technology Project 973(2014CB849801 ; NNSFC(21472010 ; NNSFC(21472010 ; 2015CB856200) ; 2015CB856200) ; 21521003 ; 21521003 ; 21561142002 ; 21561142002 ; 21625201) ; 21625201) ; National Key Research and Development Program of China(2017YFA0505200) ; National Key Research and Development Program of China(2017YFA0505200) ; National High Technology Project 973(2014CB849801 ; National High Technology Project 973(2014CB849801 ; NNSFC(21472010 ; NNSFC(21472010 ; 2015CB856200) ; 2015CB856200) ; 21521003 ; 21521003 ; 21561142002 ; 21561142002 ; 21625201) ; 21625201)
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/12786]
专题	中国科学院武汉物理与数学研究所
通讯作者	Lei, Xiaoguang; Dong, Meng-Qiu
作者单位	1.Peking Univ, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China 2.Peking Univ, Coll Chem & Mol Engn, Ctr Quantitat Biol, Beijing 100871, Peoples R China 3.NIBS, Beijing 102206, Peoples R China 4.Chinese Acad Med Sci, Beijing 100730, Peoples R China 5.Peking Union Med Coll, Grad Sch, Beijing 100730, Peoples R China 6.Peking Univ, Beijing Natl Lab Mol Sci,Minist Educ, Key Lab Bioorgan Chem & Mol Engn,Dept Chem Biol, Coll Chem & Mol Engn,Synthet & Funct Biomol Ctr, Beijing 100871, Peoples R China 7.Chinese Acad Sci, CAS Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom Mol Phys, Natl Ctr Magnet Resonance Wuhan,Wuhan Inst Phys &, Wuhan 430071, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Li, Qiang,Tan, Dan,Wang, Jian-Hua,et al. Carboxylate-Selective Chemical Cross-Linkers for Mass Spectrometric Analysis of Protein Structures[J]. ANALYTICAL CHEMISTRY,2018,90(2):1195-1201.
APA	Li, Qiang.,Tan, Dan.,Wang, Jian-Hua.,Zhang, Xiaoyun.,Li, Maodong.,...&Gong, Zhou.(2018).Carboxylate-Selective Chemical Cross-Linkers for Mass Spectrometric Analysis of Protein Structures.ANALYTICAL CHEMISTRY,90(2),1195-1201.
MLA	Li, Qiang,et al."Carboxylate-Selective Chemical Cross-Linkers for Mass Spectrometric Analysis of Protein Structures".ANALYTICAL CHEMISTRY 90.2(2018):1195-1201.