A pH-gated conformational switch regulates the phosphatase activity of bifunctional HisKA-family histidine kinases

doi:10.1038/s41467-017-02310-9

	A pH-gated conformational switch regulates the phosphatase activity of bifunctional HisKA-family histidine kinases
	Li, Conggang 1; Liu, Maili 1; Zhou, Pei 4; Jiang, Ling 1; Liu, Yixiang 1; Rose, Joshua 4; Huang, Shaojia 2,3; Hu, Yangbo 3; Wu, Qiong 1; Wang, Dan 1,2
刊名	NATURE COMMUNICATIONS
	2017-12-13
卷号	8
ISSN号	2041-1723
DOI	10.1038/s41467-017-02310-9
文献子类	Article
英文摘要	Histidine kinases are key regulators in the bacterial two-component systems that mediate the cellular response to environmental changes. The vast majority of the sensor histidine kinases belong to the bifunctional HisKA family, displaying both kinase and phosphatase activities toward their substrates. The molecular mechanisms regulating the opposing activities of these enzymes are not well understood. Through a combined NMR and crystallographic study on the histidine kinase HK853 and its response regulator RR468 from Thermotoga maritima, here we report a pH-mediated conformational switch of HK853 that shuts off its phosphatase activity under acidic conditions. Such a pH-sensing mechanism is further demonstrated in the EnvZ-OmpR two-component system from Salmonella enterica in vitro and in vivo, which directly contributes to the bacterial infectivity. Our finding reveals a broadly conserved mechanism that regulates the phosphatase activity of the largest family of bifunctional histidine kinases in response to the change of environmental pH.
WOS关键词	2-COMPONENT SIGNAL-TRANSDUCTION ; NMR-SPECTROSCOPY ; F-19 NMR ; ESCHERICHIA-COLI ; SSRA-SSRB ; SYSTEM ; OMPR ; EXCHANGE ; ENVZ ; PHOSPHORYLATION
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000417884500010
资助机构	U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences(W-31-109-Eng-38) ; U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences(W-31-109-Eng-38) ; National Key R&D Program of China(2017YFA0505400) ; National Key R&D Program of China(2017YFA0505400) ; Ministry of Science and Technology of China(2013CB910200) ; Ministry of Science and Technology of China(2013CB910200) ; Natural Science Foundation of China(21573280 ; Natural Science Foundation of China(21573280 ; National Institute of General Medical Sciences(GM115355) ; National Institute of General Medical Sciences(GM115355) ; 21603268) ; 21603268) ; U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences(W-31-109-Eng-38) ; U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences(W-31-109-Eng-38) ; National Key R&D Program of China(2017YFA0505400) ; National Key R&D Program of China(2017YFA0505400) ; Ministry of Science and Technology of China(2013CB910200) ; Ministry of Science and Technology of China(2013CB910200) ; Natural Science Foundation of China(21573280 ; Natural Science Foundation of China(21573280 ; National Institute of General Medical Sciences(GM115355) ; National Institute of General Medical Sciences(GM115355) ; 21603268) ; 21603268) ; U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences(W-31-109-Eng-38) ; U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences(W-31-109-Eng-38) ; National Key R&D Program of China(2017YFA0505400) ; National Key R&D Program of China(2017YFA0505400) ; Ministry of Science and Technology of China(2013CB910200) ; Ministry of Science and Technology of China(2013CB910200) ; Natural Science Foundation of China(21573280 ; Natural Science Foundation of China(21573280 ; National Institute of General Medical Sciences(GM115355) ; National Institute of General Medical Sciences(GM115355) ; 21603268) ; 21603268) ; U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences(W-31-109-Eng-38) ; U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences(W-31-109-Eng-38) ; National Key R&D Program of China(2017YFA0505400) ; National Key R&D Program of China(2017YFA0505400) ; Ministry of Science and Technology of China(2013CB910200) ; Ministry of Science and Technology of China(2013CB910200) ; Natural Science Foundation of China(21573280 ; Natural Science Foundation of China(21573280 ; National Institute of General Medical Sciences(GM115355) ; National Institute of General Medical Sciences(GM115355) ; 21603268) ; 21603268)
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/12762]
专题	中国科学院武汉物理与数学研究所
通讯作者	Jiang, Ling
作者单位	1.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Key Lab Magnet Resonance Biol Syst,Natl Ctr Magne, Wuhan 430071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Virol, Ctr Emerging Infect Dis, CAS Key Lab Special Pathogens & Biosafety, Wuhan 430071, Peoples R China 4.Duke Univ, Med Ctr, Dept Biochem, Durham, NC 27710 USA
推荐引用方式 GB/T 7714	Li, Conggang,Liu, Maili,Zhou, Pei,et al. A pH-gated conformational switch regulates the phosphatase activity of bifunctional HisKA-family histidine kinases[J]. NATURE COMMUNICATIONS,2017,8.
APA	Li, Conggang.,Liu, Maili.,Zhou, Pei.,Jiang, Ling.,Liu, Yixiang.,...&Wang, Dan.(2017).A pH-gated conformational switch regulates the phosphatase activity of bifunctional HisKA-family histidine kinases.NATURE COMMUNICATIONS,8.
MLA	Li, Conggang,et al."A pH-gated conformational switch regulates the phosphatase activity of bifunctional HisKA-family histidine kinases".NATURE COMMUNICATIONS 8(2017).