Development of a multiple immunoaffinity column for simultaneous determination of multiple mycotoxins in feeds using UPLC-MS/MS

doi:10.1007/s00216-016-9626-5

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 磁共振基础研究部

	Development of a multiple immunoaffinity column for simultaneous determination of multiple mycotoxins in feeds using UPLC-MS/MS
	Hu, Xiaofeng 1,2,3,4; Wang, Min 5; Zhang, Qi 2,4; Li, Peiwu 1,2,3,4; Zhang, Zhaowei 2,3,4; Hu, Rui 5
刊名	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
	2016-09-01
卷号	408 期号:22 页码:6027-6036
关键词	Multiple Mycotoxins Feed Simultaneous Determination Multiple Immunoaffinity Columns Uplc-ms/ms
DOI	10.1007/s00216-016-9626-5
文献子类	Article
英文摘要	A sensitive and specific immunoaffinity column to clean up and isolate multiple mycotoxins was developed along with a rapid one-step sample preparation procedure for ultra-performance liquid chromatography-tandem mass spectrometry analysis. Monoclonal antibodies against aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, zearalenone, ochratoxin A, sterigmatocystin, and T-2 toxin were coupled to microbeads for mycotoxin purification. We optimized a homogenization and extraction procedure as well as column loading and elution conditions to maximize recoveries from complex feed matrices. This method allowed rapid, simple, and simultaneous determination of mycotoxins in feeds with a single chromatographic run. Detection limits for these toxins ranged from 0.006 to 0.12 ng mL(-1), and quantitation limits ranged from 0.06 to 0.75 ng mL(-1). Concentration curves were linear from 0.12 to 40 mu g kg(-1) with correlation coefficients of R-2>0.99. Intra-assay and inter-assay comparisons indicated excellent repeatability and reproducibility of the multiple immunoaffinity columns. As a proof of principle, 80 feed samples were tested and several contained multiple mycotoxins. This method is sensitive, rapid, and durable enough for multiple mycotoxin determinations that fulfill European Union and Chinese testing criteria.
WOS关键词	TANDEM MASS-SPECTROMETRY ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; AFLATOXIN B-1 ; ZEARALENONE ; EXTRACTION ; METABOLISM ; OCHRATOXIN ; TOXICITY ; CLEANUP ; SAMPLES
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
WOS记录号	WOS:000382681400010
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/11504]
专题	武汉物理与数学研究所_磁共振基础研究部
作者单位	1.Minist Agr, Lab Risk Assessment Oilseeds Prod Wuhan, Wuhan 430062, Peoples R China 2.Chinese Acad Agr Sci, Oil Crops Res Inst, Wuhan 430062, Peoples R China 3.Minist Agr, Key Lab Biol & Genet Improvement Oil Crops, Wuhan 430062, Peoples R China 4.Minist Agr, Key Lab Detect Mycotoxins, Wuhan 430062, Peoples R China 5.Chinese Acad Sci, State Key Lab Magnet Resonance & Atom Mol Phys, Wuhan Inst Phys & Math, Inst Hydrobiol, 7 Donghu Rd, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Hu, Xiaofeng,Wang, Min,Zhang, Qi,et al. Development of a multiple immunoaffinity column for simultaneous determination of multiple mycotoxins in feeds using UPLC-MS/MS[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2016,408(22):6027-6036.
APA	Hu, Xiaofeng,Wang, Min,Zhang, Qi,Li, Peiwu,Zhang, Zhaowei,&Hu, Rui.(2016).Development of a multiple immunoaffinity column for simultaneous determination of multiple mycotoxins in feeds using UPLC-MS/MS.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,408(22),6027-6036.
MLA	Hu, Xiaofeng,et al."Development of a multiple immunoaffinity column for simultaneous determination of multiple mycotoxins in feeds using UPLC-MS/MS".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 408.22(2016):6027-6036.