NMR-based Metabolomics Analysis of Liver from C57BL/6 Mouse Exposed to Ionizing Radiation

doi:10.1667/RR14602.1

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 磁共振基础研究部

	NMR-based Metabolomics Analysis of Liver from C57BL/6 Mouse Exposed to Ionizing Radiation
	Hu, Jian Zhi 1; Zhang, Xu 2; Hu, Mary 1; Xiao, Xiongjie 1,2,3
刊名	RADIATION RESEARCH
	2017-07-01
卷号	188 期号:1 页码:44-55
DOI	10.1667/RR14602.1
文献子类	Article
英文摘要	The effects of ionizing radiation to human health are of great concern in the field of space exploration and for patients considering radiotherapy. However, to date, the effect of high-dose radiation on metabolism in the liver has not been clearly defined. In this study, H-1 nuclear magnetic resonance (NMR)-based metabolomics combined with multivariate data analysis was applied to study the changes of metabolism in the liver of C57BL/6 mouse after whole-body gamma (3.0 and 7.8 Gy) or proton (3.0 Gy) irradiation. Principal component analysis (PCA) and orthogonal projection to latent structures analysis (OPLS) were used for classification and identification of potential biomarkers associated with exposure to gamma and proton radiation. The results show that the radiation exposed groups can be well separated from the control group. Where the same dose was received, the proton exposed group was nevertheless well separated from the gamma-exposed group, indicating that different radiation sources induce different alterations in the metabolic profile. Common among all high-dose gamma and proton exposed groups were the statistically decreased concentrations of choline, O-phosphocholine and trimethylamine N-oxide, while the concentrations of glutamine, glutathione, malate, creatinine, phosphate, betaine and 4-hydroxyphenylacetate were statistically and significantly elevated. Since these altered metabolites are associated with multiple biological pathways, the results suggest that radiation induces abnormality in multiple biological pathways. In particular, metabolites such as 4-hydroxyphenylacetate, betaine, glutamine, choline and trimethylamine N-oxide may be prediagnostic biomarkers candidates for ionizing exposure of the liver. (C) 2017 by Radiation Research Society
WOS关键词	H-1-NMR METABOLOMICS ; PROTON-RADIATION ; SYSTEMS BIOLOGY ; GAMMA ; MICE ; METABOLISM ; IDENTIFICATION ; METABONOMICS ; BIOMARKERS ; BETAINE
WOS研究方向	Life Sciences & Biomedicine - Other Topics ; Biophysics ; Radiology, Nuclear Medicine & Medical Imaging
语种	英语
WOS记录号	WOS:000404843400004
资助机构	National Institute of Environmental Health Sciences of the National Institute of Health (NIH)(R01ES022176) ; National Institute of Environmental Health Sciences of the National Institute of Health (NIH)(R01ES022176) ; NASA Space Radiation Program(NNX07AU44G) ; NASA Space Radiation Program(NNX07AU44G) ; Dept. of Energy (DOE) Office of Biological and Environmental Research, and located at Pacific Northwest National Laboratory (PNNL) ; Dept. of Energy (DOE) Office of Biological and Environmental Research, and located at Pacific Northwest National Laboratory (PNNL) ; DOE by Battelle Memorial Institute(DE-AC06-76RLO 1830) ; DOE by Battelle Memorial Institute(DE-AC06-76RLO 1830) ; National Institute of Environmental Health Sciences of the National Institute of Health (NIH)(R01ES022176) ; National Institute of Environmental Health Sciences of the National Institute of Health (NIH)(R01ES022176) ; NASA Space Radiation Program(NNX07AU44G) ; NASA Space Radiation Program(NNX07AU44G) ; Dept. of Energy (DOE) Office of Biological and Environmental Research, and located at Pacific Northwest National Laboratory (PNNL) ; Dept. of Energy (DOE) Office of Biological and Environmental Research, and located at Pacific Northwest National Laboratory (PNNL) ; DOE by Battelle Memorial Institute(DE-AC06-76RLO 1830) ; DOE by Battelle Memorial Institute(DE-AC06-76RLO 1830)
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/11466]
专题	武汉物理与数学研究所_磁共振基础研究部
作者单位	1.Pacific Northwest Natl Lab, Richland, WA 99352 USA 2.Chinese Acad Sci, Wuhan Inst Phys & Math, Natl Ctr Magnet Resonance Wuhan, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Hu, Jian Zhi,Zhang, Xu,Hu, Mary,et al. NMR-based Metabolomics Analysis of Liver from C57BL/6 Mouse Exposed to Ionizing Radiation[J]. RADIATION RESEARCH,2017,188(1):44-55.
APA	Hu, Jian Zhi,Zhang, Xu,Hu, Mary,&Xiao, Xiongjie.(2017).NMR-based Metabolomics Analysis of Liver from C57BL/6 Mouse Exposed to Ionizing Radiation.RADIATION RESEARCH,188(1),44-55.
MLA	Hu, Jian Zhi,et al."NMR-based Metabolomics Analysis of Liver from C57BL/6 Mouse Exposed to Ionizing Radiation".RADIATION RESEARCH 188.1(2017):44-55.