Simultaneous Determination of Arginine and Citrulline in Gourd Fruits and Melons by High Performance Liquid Chromatography with Electrochemical Detection

doi:10.1007/s12161-016-0694-0

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	Simultaneous Determination of Arginine and Citrulline in Gourd Fruits and Melons by High Performance Liquid Chromatography with Electrochemical Detection
	Wang, Jie 2; Yao, Jiao 3; Deng, Yinghua 3; Chen, Rongxiang 1; Hu, Ting 2
刊名	FOOD ANALYTICAL METHODS
	2017-05-01
卷号	10 期号:5 页码:1369-1376
关键词	Citrulline Arginine High Performance Liquid Chromatography Electrochemical Detection Pre-column Derivatization
DOI	10.1007/s12161-016-0694-0
文献子类	Article
英文摘要	In this study, electrochemical detection (ECD) was for the first time coupled to high performance liquid chromatography for the analysis of arginine (Arg) and citrulline (Cit) using O-phthalaldehyde as a pre-column derivatization reagent. The chromatographic conditions and ECD parameters were systemically optimized and the herein built method demonstrated high sensitivity, ease of operation and good reproducibility. Arg and Cit were separated within 9.5 min and quantitated, giving a linear range of 0.01-10.00 mu M. The linear coefficient for the two amino acids were 0.9999 (Arg) and 0.9998 (Cit), respectively. This analytical approach adds a new tool to simultaneous determination of the two amino acids. It was applied to food sample analysis in loofah and pumpkin, and quantitation in watermelon.
WOS关键词	PHTHALALDEHYDE-SULFITE DERIVATIZATION ; AMINO-ACID NEUROTRANSMITTERS ; PRECOLUMN DERIVATIZATION ; BIOLOGICAL SAMPLES ; GC-MS ; PLASMA ; ELECTROPHORESIS ; HOMOARGININE ; METABOLITES ; VALIDATION
WOS研究方向	Food Science & Technology
语种	英语
WOS记录号	WOS:000399014600024
资助机构	National Natural Science Foundation of China(21405036) ; National Natural Science Foundation of China(21405036) ; Hubei Chenguang Talented Youth Development Foundation ; Hubei Chenguang Talented Youth Development Foundation ; Research Foundation of Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education)(ACBM 2012003) ; Research Foundation of Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education)(ACBM 2012003) ; National Natural Science Foundation of China(21405036) ; National Natural Science Foundation of China(21405036) ; Hubei Chenguang Talented Youth Development Foundation ; Hubei Chenguang Talented Youth Development Foundation ; Research Foundation of Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education)(ACBM 2012003) ; Research Foundation of Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education)(ACBM 2012003)
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/11296]
专题	武汉物理与数学研究所_磁共振应用研究部
作者单位	1.Zunyi Med Univ, Res Ctr Med & Biol, Zunyi 563003, Guizhou, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan 430071, Hubei, Peoples R China 3.Hubei Univ Educ, Dept Chem & Life Sci, Wuhan 430205, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Wang, Jie,Yao, Jiao,Deng, Yinghua,et al. Simultaneous Determination of Arginine and Citrulline in Gourd Fruits and Melons by High Performance Liquid Chromatography with Electrochemical Detection[J]. FOOD ANALYTICAL METHODS,2017,10(5):1369-1376.
APA	Wang, Jie,Yao, Jiao,Deng, Yinghua,Chen, Rongxiang,&Hu, Ting.(2017).Simultaneous Determination of Arginine and Citrulline in Gourd Fruits and Melons by High Performance Liquid Chromatography with Electrochemical Detection.FOOD ANALYTICAL METHODS,10(5),1369-1376.
MLA	Wang, Jie,et al."Simultaneous Determination of Arginine and Citrulline in Gourd Fruits and Melons by High Performance Liquid Chromatography with Electrochemical Detection".FOOD ANALYTICAL METHODS 10.5(2017):1369-1376.