Solution NMR structure of zinc finger 4 and 5 from human INSM1, an essential regulator of neuroendocrine differentiation

doi:10.1002/prot.25259

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 磁共振基础研究部

	Solution NMR structure of zinc finger 4 and 5 from human INSM1, an essential regulator of neuroendocrine differentiation
	Wang, Huapu 1,2; Zhu, Jiang 2; Yang, Yunhuang 2; Liu, Maili 2; Kennedy, Michael A.3,4; Ramelot, Theresa A.3,4; Yue, Xiali 1; Hu, Rui 2
刊名	PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
	2017-05-01
卷号	85 期号:5 页码:957-962
关键词	Insm1 Zf4-5 Consensus Dna Solution Structure Mechanism
DOI	10.1002/prot.25259
文献子类	Article
英文摘要	Human INSM1 containing five C-terminal C2H2-type zinc fingers (ZFs), is a key regulator of neuroendocrine development. Previous research reported that full-length INSM1 containing all five ZFs recognized a consensus DNA sequence. Structure elucidation of human INSM1 ZFs is currently insufficient to understand the DNA binding mechanism. Herein, we present the solution NMR structure of ZF4-5, in which the two ZFs adopt a head-to-tail arrangement and each ZF features a canonical fold. NMR titrations and isothermal titration calorimetry experiments showed that ZF4-5 binds weakly to the consensus DNA sequence. Proteins 2017; 85:957-962. (c) 2016 Wiley Periodicals, Inc.
WOS关键词	DNA-BINDING DOMAIN ; PROTEIN STRUCTURES
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
语种	英语
WOS记录号	WOS:000399380400015
资助机构	National Institute of General Medical Sciences of USA(U54-GM074958 ; National Institute of General Medical Sciences of USA(U54-GM074958 ; Ministry of Science and Technology(2016YFA051201) ; Ministry of Science and Technology(2016YFA051201) ; National Natural Sciences Foundation of China(21575155) ; National Natural Sciences Foundation of China(21575155) ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; U54-GM094597) ; U54-GM094597) ; National Institute of General Medical Sciences of USA(U54-GM074958 ; National Institute of General Medical Sciences of USA(U54-GM074958 ; Ministry of Science and Technology(2016YFA051201) ; Ministry of Science and Technology(2016YFA051201) ; National Natural Sciences Foundation of China(21575155) ; National Natural Sciences Foundation of China(21575155) ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; U54-GM094597) ; U54-GM094597)
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/11294]
专题	武汉物理与数学研究所_磁共振基础研究部
作者单位	1.Huazhong Agr Univ, Dept Chem, Coll Sci, Wuhan 430070, Peoples R China 2.Chinese Acad Sci, State Key Lab Magnet Resonance & Atom Mol Phys, Wuhan Ctr Magnet Resonance, Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China 3.Miami Univ, Dept Chem & Biochem, Oxford, OH 45056 USA 4.Miami Univ, Northeast Struct Genom Consortium, Oxford, OH 45056 USA
推荐引用方式 GB/T 7714	Wang, Huapu,Zhu, Jiang,Yang, Yunhuang,et al. Solution NMR structure of zinc finger 4 and 5 from human INSM1, an essential regulator of neuroendocrine differentiation[J]. PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS,2017,85(5):957-962.
APA	Wang, Huapu.,Zhu, Jiang.,Yang, Yunhuang.,Liu, Maili.,Kennedy, Michael A..,...&Hu, Rui.(2017).Solution NMR structure of zinc finger 4 and 5 from human INSM1, an essential regulator of neuroendocrine differentiation.PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS,85(5),957-962.
MLA	Wang, Huapu,et al."Solution NMR structure of zinc finger 4 and 5 from human INSM1, an essential regulator of neuroendocrine differentiation".PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS 85.5(2017):957-962.