Rapid determination of gizzerosine in fish meals using microchip capillary electrophoresis with laser-induced fluorescence detection

doi:10.1080/19440049.2017.1292053

	Rapid determination of gizzerosine in fish meals using microchip capillary electrophoresis with laser-induced fluorescence detection
	Xu, PL; Yang, L; Liu, YM; Liao, X; Liao, X (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Chengdu, Peoples R China.; Xiao, MW; Bai, XL; Zhao, Y
刊名	FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
	2017
卷号	34 期号:5 页码:760-765
关键词	Microchip Capillary Electrophoresis Laser-induced Fluorescence Gizzerosine Surface Coating Poultry Feeds
DOI	10.1080/19440049.2017.1292053
产权排序	1
文献子类	Article
英文摘要	Sensitive detection of gizzerosine, a causative agent for deadly gizzard erosion in chicken feeds, is very important to the poultry industry. In this work, a new method was developed based on microchip capillary electrophoresis (MCE) with laser-induced fluorescence (LIF) detection for rapid analysis of gizzerosine, a biogenic amine in fish meals. The MCE separation was performed on a glass microchip using sodium dodecyl sulfate (SDS) as dynamic coating modifier. Separation conditions, including running buffer pH and concentration, SDS concentration, and the separation voltage were investigated to achieve fast and sensitive quantification of gizzerosine. The assay proposed was very quick and could be completed within 65s. A linear calibration curve was obtained in the range from 0.04 to 1.8gml(-1) gizzerosine. The detection limit was 0.025gml(-1) (0.025mgkg(-1)), which was far more sensitive than those previously reported. Gizzerosine was well separated from other endogenous components in fish meal samples. Recovery of gizzerosine from this sample matrix (n=3) was determined to be 97.2-102.8%. The results from analysing fish meal samples indicated that the present MCE-LIF method might hold the potential for rapid detection of gizzerosine in poultry feeds.
学科主题	Chemistry ; Food Science & Technology ; Toxicology
语种	英语
资助机构	This work was supported by the State Key Laboratory of Phytochemistry and Plant Resources in West China [grant number P2015-KF12]; the West Light Foundation of The Chinese Academy of Sciences; and the National Natural Science Foundation of China [grant number 81173536]. ; State Key Laboratory of Phytochemistry and Plant Resources in West China [P2015-KF12] ; This work was supported by the State Key Laboratory of Phytochemistry and Plant Resources in West China [grant number P2015-KF12]; the West Light Foundation of The Chinese Academy of Sciences; and the National Natural Science Foundation of China [grant number 81173536]. ; State Key Laboratory of Phytochemistry and Plant Resources in West China [P2015-KF12] ; West Light Foundation of The Chinese Academy of Sciences ; West Light Foundation of The Chinese Academy of Sciences ; National Natural Science Foundation of China [81173536] ; National Natural Science Foundation of China [81173536] ; This work was supported by the State Key Laboratory of Phytochemistry and Plant Resources in West China [grant number P2015-KF12]; the West Light Foundation of The Chinese Academy of Sciences; and the National Natural Science Foundation of China [grant number 81173536]. ; State Key Laboratory of Phytochemistry and Plant Resources in West China [P2015-KF12] ; This work was supported by the State Key Laboratory of Phytochemistry and Plant Resources in West China [grant number P2015-KF12]; the West Light Foundation of The Chinese Academy of Sciences; and the National Natural Science Foundation of China [grant number 81173536]. ; State Key Laboratory of Phytochemistry and Plant Resources in West China [P2015-KF12] ; West Light Foundation of The Chinese Academy of Sciences ; West Light Foundation of The Chinese Academy of Sciences ; National Natural Science Foundation of China [81173536] ; National Natural Science Foundation of China [81173536]
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/29260]
专题	成都生物研究所_天然产物研究
通讯作者	Liao, X (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Chengdu, Peoples R China.
推荐引用方式 GB/T 7714	Xu, PL,Yang, L,Liu, YM,et al. Rapid determination of gizzerosine in fish meals using microchip capillary electrophoresis with laser-induced fluorescence detection[J]. FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT,2017,34(5):760-765.
APA	Xu, PL.,Yang, L.,Liu, YM.,Liao, X.,Liao, X .,...&Zhao, Y.(2017).Rapid determination of gizzerosine in fish meals using microchip capillary electrophoresis with laser-induced fluorescence detection.FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT,34(5),760-765.
MLA	Xu, PL,et al."Rapid determination of gizzerosine in fish meals using microchip capillary electrophoresis with laser-induced fluorescence detection".FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT 34.5(2017):760-765.