Weigh Biomaterials by Quantifying Species-specific DNA with Real-time PCR

doi:10.1038/s41598-017-05083-9

	Weigh Biomaterials by Quantifying Species-specific DNA with Real-time PCR
	Chen, R; Wang, JY; Yuan, Y; Deng, Y; Lai, XR; Du, F; Dong, J; Huang, X; Cui, X; Tang, Z
刊名	SCIENTIFIC REPORTS
	2017
卷号	7 页码:4774（1-7）
DOI	10.1038/s41598-017-05083-9
产权排序	1
文献子类	Article
英文摘要	What's on the label is not what's in the bottle, from food products to herbal medicinal products (HMPs), economically-motivated biomaterials adulteration is a long-term problem affecting the food and drug industry. Accurate identification of the biomaterial ingredients in processed commodities is highly desirable. In this field, DNA-based techniques have proved to be powerful tools to overcome qualitative challenges. However, is it possible to quantify the weight of biological materials with PCR? Therefore, a basic scientific question needs to be answered: what's the relationship between DNA content and the mass of biological materials? Is DNA content directly proportional to the mass of biological materials as most of the researchers previously thought? In this study, we firstly found that there exists a linear relation between DNA contents and the weight of biomaterials indeed when the analytical practices are fully controlled. In this case, the mass of targeted biomaterials in the highly processed commercial products can also be calculated by quantifying the species-specific DNA through classic real-time PCR with a good reproducibility.
学科主题	Science & Technology - Other Topics
语种	英语
资助机构	This study was supported by National Natural Science Foundation of China (Grant No. 81403185, 21402189, 81373961 and 21572222); West Light Foundation of The Chinese Academy of Sciences (Y4C3021100); Science and technology project of Sichuan Province (2014RZ0022). ; National Natural Science Foundation of China [81403185, 21402189, 81373961, 21572222] ; This study was supported by National Natural Science Foundation of China (Grant No. 81403185, 21402189, 81373961 and 21572222); West Light Foundation of The Chinese Academy of Sciences (Y4C3021100); Science and technology project of Sichuan Province (2014RZ0022). ; National Natural Science Foundation of China [81403185, 21402189, 81373961, 21572222] ; West Light Foundation of The Chinese Academy of Sciences [Y4C3021100] ; West Light Foundation of The Chinese Academy of Sciences [Y4C3021100] ; Science and technology project of Sichuan Province [2014RZ0022] ; Science and technology project of Sichuan Province [2014RZ0022] ; This study was supported by National Natural Science Foundation of China (Grant No. 81403185, 21402189, 81373961 and 21572222); West Light Foundation of The Chinese Academy of Sciences (Y4C3021100); Science and technology project of Sichuan Province (2014RZ0022). ; National Natural Science Foundation of China [81403185, 21402189, 81373961, 21572222] ; This study was supported by National Natural Science Foundation of China (Grant No. 81403185, 21402189, 81373961 and 21572222); West Light Foundation of The Chinese Academy of Sciences (Y4C3021100); Science and technology project of Sichuan Province (2014RZ0022). ; National Natural Science Foundation of China [81403185, 21402189, 81373961, 21572222] ; West Light Foundation of The Chinese Academy of Sciences [Y4C3021100] ; West Light Foundation of The Chinese Academy of Sciences [Y4C3021100] ; Science and technology project of Sichuan Province [2014RZ0022] ; Science and technology project of Sichuan Province [2014RZ0022]
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/29225]
专题	成都生物研究所_天然产物研究
通讯作者	Tang, Z (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Nat Prod Res Ctr, Chengdu 610041, Peoples R China.; Tang, Z (reprint author), Univ Chinese Acad Sci, Beijing 100049, Peoples R China.
推荐引用方式 GB/T 7714	Chen, R,Wang, JY,Yuan, Y,et al. Weigh Biomaterials by Quantifying Species-specific DNA with Real-time PCR[J]. SCIENTIFIC REPORTS,2017,7:4774（1-7）.
APA	Chen, R.,Wang, JY.,Yuan, Y.,Deng, Y.,Lai, XR.,...&Tang, Z .(2017).Weigh Biomaterials by Quantifying Species-specific DNA with Real-time PCR.SCIENTIFIC REPORTS,7,4774（1-7）.
MLA	Chen, R,et al."Weigh Biomaterials by Quantifying Species-specific DNA with Real-time PCR".SCIENTIFIC REPORTS 7(2017):4774（1-7）.