Flavonoid glycosides isolated from Epimedium brevicornum and their estrogen biosynthesis-promoting effects

doi:10.1038/s41598-017-08203-7

	Flavonoid glycosides isolated from Epimedium brevicornum and their estrogen biosynthesis-promoting effects
	Liu, CL; Li, F; Du, BW; Lu, DF; Wu, WX; Wongkrajang, K; Wang, L; Pu, WC; Liu, HW; Wang, MK
刊名	SCIENTIFIC REPORTS
	2017
卷号	7 页码:7760（1-12）
DOI	10.1038/s41598-017-08203-7
产权排序	1
文献子类	Article
英文摘要	Epimedium brevicornum Maxim has a long history of use in the treatment of estrogen deficiency-related diseases. However, the chemical constituents and mechanism of action of this medicinal plant are not fully understood. In the present study, we isolated four new isoprenylated flavonoid glycosides, as well as 16 known flavonoids (13 isoprenylated flavonoids), from this plant. The chemical structures of the new flavonoid glycosides were elucidated by extensive spectroscopic analysis. The new compounds 1-4 were potent promoters of estrogen biosynthesis in human ovarian granulosa-like KGN cells. ZW1, an isoprenylated flavonoid analogue and a specific inhibitor of phosphodiesterase 5 (PDE5), was synthesized and used to explore the mechanism of the isoprenylated analogues on estrogen biosynthesis. ZW1 treatment increased estrogen production by upregulation of aromatase mRNA and protein expression. ZW1 increased the phosphorylation of cAMP response element-binding protein (CREB). Further study showed that the inhibition of PDE5 by ZW1 increased estrogen biosynthesis partly through suppression of phosphodiesterase 3 (PDE3). Our results suggested that the isoprenylated flavonoids from E. brevicornum may produce beneficial health effects through the promotion of estrogen biosynthesis. PDE5 warrants further investigation as a new therapeutic target for estrogen biosynthesis in the prevention and treatment of estrogen-deficiency related diseases.
学科主题	Science & Technology - Other Topics
资助项目	Pu, Wenchen/E-9537-2018
项目编号	中国科学院山地生态恢复与生物资源利用重点实验室
语种	英语
资助机构	This work was supported by the National Natural Science Foundation of China (grants 21372214, 21561142003, 21550110193), Science & Technology Department of Sichuan Province (grants 2014JQ0028, 2016HH0073), CAS President's International Fellowship Initiative (No. 2015PB061), Research Foundation of State General Administration of The People's Republic of China for Quality Supervision and Inspection and Quarantine (No. 2015IK180), the West Light Foundation of Chinese Academy of Sciences, the Key Project of Education Department of Sichuan Province Natural Science Foundation (No. 14ZA0293), and Program of the Key Laboratory of Exploitation and Study of Distinctive Plants in Education Department of Sichuan Province (No. SCTZ201304). ; National Natural Science Foundation of China [21372214, 21561142003, 21550110193] ; This work was supported by the National Natural Science Foundation of China (grants 21372214, 21561142003, 21550110193), Science & Technology Department of Sichuan Province (grants 2014JQ0028, 2016HH0073), CAS President's International Fellowship Initiative (No. 2015PB061), Research Foundation of State General Administration of The People's Republic of China for Quality Supervision and Inspection and Quarantine (No. 2015IK180), the West Light Foundation of Chinese Academy of Sciences, the Key Project of Education Department of Sichuan Province Natural Science Foundation (No. 14ZA0293), and Program of the Key Laboratory of Exploitation and Study of Distinctive Plants in Education Department of Sichuan Province (No. SCTZ201304). ; National Natural Science Foundation of China [21372214, 21561142003, 21550110193] ; Science & Technology Department of Sichuan Province [2014JQ0028, 2016HH0073] ; Science & Technology Department of Sichuan Province [2014JQ0028, 2016HH0073] ; CAS President's International Fellowship Initiative [2015PB061] ; CAS President's International Fellowship Initiative [2015PB061] ; Research Foundation of State General Administration of The People's Republic of China for Quality Supervision and Inspection and Quarantine [2015IK180] ; Research Foundation of State General Administration of The People's Republic of China for Quality Supervision and Inspection and Quarantine [2015IK180] ; West Light Foundation of Chinese Academy of Sciences ; West Light Foundation of Chinese Academy of Sciences ; Key Project of Education Department of Sichuan Province Natural Science Foundation [14ZA0293] ; Key Project of Education Department of Sichuan Province Natural Science Foundation [14ZA0293] ; Program of the Key Laboratory of Exploitation and Study of Distinctive Plants in Education Department of Sichuan Province [SCTZ201304] ; Program of the Key Laboratory of Exploitation and Study of Distinctive Plants in Education Department of Sichuan Province [SCTZ201304] ; This work was supported by the National Natural Science Foundation of China (grants 21372214, 21561142003, 21550110193), Science & Technology Department of Sichuan Province (grants 2014JQ0028, 2016HH0073), CAS President's International Fellowship Initiative (No. 2015PB061), Research Foundation of State General Administration of The People's Republic of China for Quality Supervision and Inspection and Quarantine (No. 2015IK180), the West Light Foundation of Chinese Academy of Sciences, the Key Project of Education Department of Sichuan Province Natural Science Foundation (No. 14ZA0293), and Program of the Key Laboratory of Exploitation and Study of Distinctive Plants in Education Department of Sichuan Province (No. SCTZ201304). ; National Natural Science Foundation of China [21372214, 21561142003, 21550110193] ; This work was supported by the National Natural Science Foundation of China (grants 21372214, 21561142003, 21550110193), Science & Technology Department of Sichuan Province (grants 2014JQ0028, 2016HH0073), CAS President's International Fellowship Initiative (No. 2015PB061), Research Foundation of State General Administration of The People's Republic of China for Quality Supervision and Inspection and Quarantine (No. 2015IK180), the West Light Foundation of Chinese Academy of Sciences, the Key Project of Education Department of Sichuan Province Natural Science Foundation (No. 14ZA0293), and Program of the Key Laboratory of Exploitation and Study of Distinctive Plants in Education Department of Sichuan Province (No. SCTZ201304). ; National Natural Science Foundation of China [21372214, 21561142003, 21550110193] ; Science & Technology Department of Sichuan Province [2014JQ0028, 2016HH0073] ; Science & Technology Department of Sichuan Province [2014JQ0028, 2016HH0073] ; CAS President's International Fellowship Initiative [2015PB061] ; CAS President's International Fellowship Initiative [2015PB061] ; Research Foundation of State General Administration of The People's Republic of China for Quality Supervision and Inspection and Quarantine [2015IK180] ; Research Foundation of State General Administration of The People's Republic of China for Quality Supervision and Inspection and Quarantine [2015IK180] ; West Light Foundation of Chinese Academy of Sciences ; West Light Foundation of Chinese Academy of Sciences ; Key Project of Education Department of Sichuan Province Natural Science Foundation [14ZA0293] ; Key Project of Education Department of Sichuan Province Natural Science Foundation [14ZA0293] ; Program of the Key Laboratory of Exploitation and Study of Distinctive Plants in Education Department of Sichuan Province [SCTZ201304] ; Program of the Key Laboratory of Exploitation and Study of Distinctive Plants in Education Department of Sichuan Province [SCTZ201304]
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/29218]
专题	成都生物研究所_天然产物研究
通讯作者	Wang, MK; Wang, F (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Sichuan, Peoples R China.; Wang, MK (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Key Lab Sichuan Prov, Key Lab Mt Ecol Restorat & Bioresource Utilizat &, Chengdu 610041, Sichuan, Peoples R China.
推荐引用方式 GB/T 7714	Liu, CL,Li, F,Du, BW,et al. Flavonoid glycosides isolated from Epimedium brevicornum and their estrogen biosynthesis-promoting effects[J]. SCIENTIFIC REPORTS,2017,7:7760（1-12）.
APA	Liu, CL.,Li, F.,Du, BW.,Lu, DF.,Wu, WX.,...&Wang, MK .(2017).Flavonoid glycosides isolated from Epimedium brevicornum and their estrogen biosynthesis-promoting effects.SCIENTIFIC REPORTS,7,7760（1-12）.
MLA	Liu, CL,et al."Flavonoid glycosides isolated from Epimedium brevicornum and their estrogen biosynthesis-promoting effects".SCIENTIFIC REPORTS 7(2017):7760（1-12）.