Functionalized carbon quantum dots with dopamine for tyrosinase activity analysis

doi:10.1016/j.aca.2017.09.038

	Functionalized carbon quantum dots with dopamine for tyrosinase activity analysis
	Hu, JJ; Bai, XL; Liu, YM; Liao, X; Liao, X (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Peoples R China.; Liu, YM (reprint author), Jackson State Univ, Dept Chem & Biochem, 1400 Lynch St, Jackson, MS 39217 USA.
刊名	ANALYTICA CHIMICA ACTA
	2017
卷号	995 期号:2017 页码:99-105
关键词	Tyrosinase Carbon Quantum Dots Fluorescence Assay Photo-induced Electron Transfer
DOI	10.1016/j.aca.2017.09.038
产权排序	1
文献子类	Article
英文摘要	Tyrosinase (TYR) is a key enzyme in melanin biosynthesis and its activity is an important biomarker for dermatological disorders, such as vitiligo, melanoma and actinic damages. Sensitive assay for TYR activity is significant for basic and clinical research. In this work, a facile fluorescent assay for TYR activity based on dopamine functionalized carbon quantum dots (CQDs-Dopa) has been developed. Dopamine (Dopa) was covalently bond to CQDs through a simple one-pot hydrothermal method, and the prepared CQDs-Dopa exhibited a fluorescence emission at 499 nm under exciting wavelength at 310 nm with a quantum yield of approximately 2.1%. When TYR was mixed with CODs-Dopa, the dopamine moiety in CQDs-Dopa conjugate was oxidized to O-dopaquinone, and an intra-particle photo-induced electron transfer (PET) process consequently occurred between CQDs and O-dopaquinone to quench the fluorescence of CQDs-Dopa. TYR activity can be determined based on the fluorescence quenching degree of CQDs-Dopa. This assay covered two broad linear ranges: 44.4-711.1 U L-1 and 711.1-2925.4 U L-1, with detection limit of 17.7 U L-1. The proposed fluorescent assay was applied to TYR activity measurement in human serum samples. It showed promising potential for TYR activity assay in clinical applications. (C) 2017 Elsevier B.V. All rights reserved.
学科主题	Chemistry
语种	英语
资助机构	This work is supported by the West Light Foundation of the Chinese Academy of Sciences, the State Key Laboratory of Phytochemistry and Plant Resources in West China (No. P2015-KF12). ; West Light Foundation of the Chinese Academy of Sciences ; This work is supported by the West Light Foundation of the Chinese Academy of Sciences, the State Key Laboratory of Phytochemistry and Plant Resources in West China (No. P2015-KF12). ; West Light Foundation of the Chinese Academy of Sciences ; State Key Laboratory of Phytochemistry and Plant Resources in West China [P2015-KF12] ; State Key Laboratory of Phytochemistry and Plant Resources in West China [P2015-KF12] ; This work is supported by the West Light Foundation of the Chinese Academy of Sciences, the State Key Laboratory of Phytochemistry and Plant Resources in West China (No. P2015-KF12). ; West Light Foundation of the Chinese Academy of Sciences ; This work is supported by the West Light Foundation of the Chinese Academy of Sciences, the State Key Laboratory of Phytochemistry and Plant Resources in West China (No. P2015-KF12). ; West Light Foundation of the Chinese Academy of Sciences ; State Key Laboratory of Phytochemistry and Plant Resources in West China [P2015-KF12] ; State Key Laboratory of Phytochemistry and Plant Resources in West China [P2015-KF12]
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/29207]
专题	成都生物研究所_天然产物研究
通讯作者	Liao, X (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Peoples R China.; Liu, YM (reprint author), Jackson State Univ, Dept Chem & Biochem, 1400 Lynch St, Jackson, MS 39217 USA.
推荐引用方式 GB/T 7714	Hu, JJ,Bai, XL,Liu, YM,et al. Functionalized carbon quantum dots with dopamine for tyrosinase activity analysis[J]. ANALYTICA CHIMICA ACTA,2017,995(2017):99-105.
APA	Hu, JJ,Bai, XL,Liu, YM,Liao, X,Liao, X ,&Liu, YM .(2017).Functionalized carbon quantum dots with dopamine for tyrosinase activity analysis.ANALYTICA CHIMICA ACTA,995(2017),99-105.
MLA	Hu, JJ,et al."Functionalized carbon quantum dots with dopamine for tyrosinase activity analysis".ANALYTICA CHIMICA ACTA 995.2017(2017):99-105.