Optimisation of ultra-performance LC conditions using response surface methodology for rapid separation and quantitative determination of phenolic compounds in Artemisia minor

	Optimisation of ultra-performance LC conditions using response surface methodology for rapid separation and quantitative determination of phenolic compounds in Artemisia minor
	Zhou, Yan; Choi, Franky Fung-Kei; He, Zhi Zhou; Song, Jing-Zheng; Qiao, Chun-Feng; Liu, Xin; Ding, Li-Sheng; Gesang, Suo-Lang; Xu, Hong-Xi; Xu, HX, Shanghai Univ Tradit Chinese Med, Shanghai, Peoples R China
刊名	JOURNAL OF SEPARATION SCIENCE
	2010
卷号	33 期号:23-24 页码:3675-3682
关键词	Artemisia Minor Box-behnken Designs (Bbds) Phenolic Compounds Quadrupole-tof-ms Ultra-performance Lc
ISSN号	1615-9306
产权排序	1
英文摘要	A method that couples rapid, sensitive, reproducible and accurate ultra-performance LC (UPLC) with quadrupole-TOF-MS was established for the first simultaneous qualitative and quantitative analysis of phenolic compounds in Artemisia minor. Box-Behnken designs (BBDs) were applied as an effective tool to optimise major parameters that influence the resolution of UPLC, including three gradient steps and column temperature. Under optimal UPLC conditions, a total of 23 phenolic compounds in the crude methanol extracts of A. minor were well separated on a Waters Acquity UPLC BEH C-18 column (100 x 2.1 mm, 1.7 mu m particle size) within 16.5 min, and the compounds were unequivocally or tentatively identified via comparisons with authentic standards and literature. In this study, a total of six major phenolic compounds were quantified in A. minor and the method was validated to be sensitive, precise and accurate within the LOD from 1.24 to 5.27 mu g/mL, and the overall intra-and inter-day variations in detection were less than 3.76%. The recovery of the method ranged from 97.9 to 103.8% with RSDs that were less than 5.8%. These results demonstrate that this approach has the potential for quality control of A. minor and other Tibetan herbal medicines.
学科主题	Chemistry, Analytical
资助项目	中国科学院山地生态恢复与生物资源利用重点实验室
语种	英语
资助机构	National Key Technology R&D Program of China [2007BAI31B02]; National Natural Science Foundation of China [30973634, 21072185] ; National Key Technology R&D Program of China [2007BAI31B02]; National Natural Science Foundation of China [30973634, 21072185] ; National Key Technology R&D Program of China [2007BAI31B02]; National Natural Science Foundation of China [30973634, 21072185] ; National Key Technology R&D Program of China [2007BAI31B02]; National Natural Science Foundation of China [30973634, 21072185]
公开日期	2011-07-08
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/22484]
专题	成都生物研究所_生态研究
通讯作者	Xu, HX, Shanghai Univ Tradit Chinese Med, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Yan,Choi, Franky Fung-Kei,He, Zhi Zhou,et al. Optimisation of ultra-performance LC conditions using response surface methodology for rapid separation and quantitative determination of phenolic compounds in Artemisia minor[J]. JOURNAL OF SEPARATION SCIENCE,2010,33(23-24):3675-3682.
APA	Zhou, Yan.,Choi, Franky Fung-Kei.,He, Zhi Zhou.,Song, Jing-Zheng.,Qiao, Chun-Feng.,...&Xu, HX, Shanghai Univ Tradit Chinese Med, Shanghai, Peoples R China.(2010).Optimisation of ultra-performance LC conditions using response surface methodology for rapid separation and quantitative determination of phenolic compounds in Artemisia minor.JOURNAL OF SEPARATION SCIENCE,33(23-24),3675-3682.
MLA	Zhou, Yan,et al."Optimisation of ultra-performance LC conditions using response surface methodology for rapid separation and quantitative determination of phenolic compounds in Artemisia minor".JOURNAL OF SEPARATION SCIENCE 33.23-24(2010):3675-3682.