Detection of carbonyl groups in triterpenoids by hydroxylamine hydrochloride derivatization using electrospray ionization mass spectrometry

	Detection of carbonyl groups in triterpenoids by hydroxylamine hydrochloride derivatization using electrospray ionization mass spectrometry
	Liu, X; Zhou, Y; Chen, HB; Peng, SL; Gu, YC; Ding, LS; Zhou, Y, Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Sichuan, Peoples R China.
刊名	RAPID COMMUNICATIONS IN MASS SPECTROMETRY
	2008
卷号	22 期号:12 页码:1981_1992
ISSN号	0951-4198
产权排序	1
英文摘要	A rapid, simple electrospray ionization mass spectrometry (ESI-MS) sensitivity enhancement method for detecting carbonyl groups in triterpenoids has been developed by using hydroxylamine hydrochloride (NH2OH center dot HCl) as a derivatization reagent. We use the oxime formed during the derivatization reactions and its Beckmann rearrangement intermediates as a means of detecting the carbonyl groups originally present in these triterpenoids. In comparison with other derivatization methods reported in the literature, this method is simple, specific and can be used to detect carbonyl groups in triterpenoids which have low polarity and are poorly or non-ionizable. Moreover, it can also be used to detect hydroxyl groups by using the Dess-Martin periodinane (DMP) to convert primary and secondary hydroxyls into carbonyl groups. Copyright (C) 2008 John Wiley & Sons, Ltd.
学科主题	Biochemical Research Methods ; Chemistry, Analytical ; Spectroscopy
语种	英语
WOS记录号	WOS:000256985100025
公开日期	2011-07-08
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/18079]
专题	成都生物研究所_天然产物研究
通讯作者	Zhou, Y, Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Sichuan, Peoples R China.
推荐引用方式 GB/T 7714	Liu, X,Zhou, Y,Chen, HB,et al. Detection of carbonyl groups in triterpenoids by hydroxylamine hydrochloride derivatization using electrospray ionization mass spectrometry[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2008,22(12):1981_1992.
APA	Liu, X.,Zhou, Y.,Chen, HB.,Peng, SL.,Gu, YC.,...&Zhou, Y, Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Sichuan, Peoples R China..(2008).Detection of carbonyl groups in triterpenoids by hydroxylamine hydrochloride derivatization using electrospray ionization mass spectrometry.RAPID COMMUNICATIONS IN MASS SPECTROMETRY,22(12),1981_1992.
MLA	Liu, X,et al."Detection of carbonyl groups in triterpenoids by hydroxylamine hydrochloride derivatization using electrospray ionization mass spectrometry".RAPID COMMUNICATIONS IN MASS SPECTROMETRY 22.12(2008):1981_1992.