3D-Bioprinted Osteoblast-Laden Nanocomposite Hydrogel Constructs with Induced Microenvironments Promote Cell Viability, Differentiation, and Osteogenesis both In Vitro and In Vivo

	3D-Bioprinted Osteoblast-Laden Nanocomposite Hydrogel Constructs with Induced Microenvironments Promote Cell Viability, Differentiation, and Osteogenesis both In Vitro and In Vivo
	Xinyun Zhai; Changshun Ruan; Yufei Ma; Delin Cheng; Mingming Wu; Wenguang Liu; Xiaoli Zhao; Haobo Pan; William Weijia Lu
刊名	Advanced Science
	2017
文献子类	期刊论文
英文摘要	An osteoblast-laden nanocomposite hydrogel construct, based on polyethylene glycol diacrylate (PEGDA)/laponite XLG nanoclay (Clay)/hyaluronic acid sodium salt (HA) bio-inks, was developed by a two-channel three-dimensional bioprinting (3D-bioprinting) method. The novel biodegradable bio-ink A, comprised of a PEG-Clay nanocomposite crosslinked hydrogel, was used to facilitate 3D-bioprinting and enabled the efficient delivery of oxygen and nutrients to growing cells. HA with encapsulated primary rat osteoblasts (ROBs) was applied as bio-ink B with a view to improving cell viability, distribution uniformity, and deposition efficiency. The cell-laden PEG-Clay constructs not only encapsulated osteoblasts with more than 95% viability in the short term, but also exhibited excellent osteogenic ability in the long term, due to the release of bioactive ions (magnesium ions, Mg2+ and silicon ions, Si4+), which induced the suitable microenvironment to promote the differentiation of the loaded exogenous ROBs, both in vitro and in vivo. This 3D-bioprinting method holds much promise for bone tissue regeneration in terms of cell engraftment, survival, and ultimately long-term function.
URL标识	查看原文
语种	英语
WOS记录号	WOS:000428310500002
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/12374]
专题	深圳先进技术研究院_医药所
作者单位	Advanced Science
推荐引用方式 GB/T 7714	Xinyun Zhai,Changshun Ruan,Yufei Ma,et al. 3D-Bioprinted Osteoblast-Laden Nanocomposite Hydrogel Constructs with Induced Microenvironments Promote Cell Viability, Differentiation, and Osteogenesis both In Vitro and In Vivo[J]. Advanced Science,2017.
APA	Xinyun Zhai.,Changshun Ruan.,Yufei Ma.,Delin Cheng.,Mingming Wu.,...&William Weijia Lu.(2017).3D-Bioprinted Osteoblast-Laden Nanocomposite Hydrogel Constructs with Induced Microenvironments Promote Cell Viability, Differentiation, and Osteogenesis both In Vitro and In Vivo.Advanced Science.
MLA	Xinyun Zhai,et al."3D-Bioprinted Osteoblast-Laden Nanocomposite Hydrogel Constructs with Induced Microenvironments Promote Cell Viability, Differentiation, and Osteogenesis both In Vitro and In Vivo".Advanced Science (2017).