Inhibition of TRPC3 downregulates airway hyperresponsiveness, remodeling of OVA-sensitized mouse.

	Inhibition of TRPC3 downregulates airway hyperresponsiveness, remodeling of OVA-sensitized mouse.
	Qiu, Chen; Wang, Lingwei; Li, Jie; Zhang, Jian; He, Qi; Weng, Xuanwen; Huang, Yanmei; Guan, Minjie
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2017
文献子类	期刊论文
英文摘要	Airway hyperresponsiveness (AHR), airway remodeling and inflammation are the fundamental pathological alterations that occur in asthma. Transient receptor potential canonical 3 (TRPC3) has been implicated in diverse functions of airway smooth muscle cells (ASMCs) in asthma. However, the underlying mechanisms remain incompletely understood. We investigated the mRNA and protein expression of TRPC3 in ASMCs from normal and OVA-sensitized mouse. And the effects of inhibition or knockdown of TRPC3 with Ethyl-1-(4-(2,3,3-trichloroacrylamide) phenyl) 5-(trifluoromethyl)-1H -pyrazole-4-carboxylate (Pyr3) and lentiviral shRNA on OVA-sensitized mouse AHR, airway remodeling, circulating inflammatory cytokines, cell proliferation and migration. We found that TRPC3 mRNA and protein expression levels were significantly increased in ASMCs from OVA-sensitized mouse. Inhibiting TRPC3 with continuous subcutaneous administration of Pyr3 decreased enhanced pause (Penh) of OVA sensitized mouse. Meanwhile, both Pyr3 and lentiviral shRNA treatment of ASMCs in OVA-sensitized mouse significantly decreased their proliferation and migration. These results suggest that TRPC3 plays a critical role in asthma and represents a promising new target for asthma treatment. (C) 2016 Elsevier Inc. All rights reserved.
URL标识	查看原文
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/12339]
专题	深圳先进技术研究院_医药所
作者单位	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
推荐引用方式 GB/T 7714	Qiu, Chen,Wang, Lingwei,Li, Jie,et al. Inhibition of TRPC3 downregulates airway hyperresponsiveness, remodeling of OVA-sensitized mouse.[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2017.
APA	Qiu, Chen.,Wang, Lingwei.,Li, Jie.,Zhang, Jian.,He, Qi.,...&Guan, Minjie.(2017).Inhibition of TRPC3 downregulates airway hyperresponsiveness, remodeling of OVA-sensitized mouse..BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.
MLA	Qiu, Chen,et al."Inhibition of TRPC3 downregulates airway hyperresponsiveness, remodeling of OVA-sensitized mouse.".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2017).