A novel bone targeting delivery system carrying phytomolecule icaritin for prevention of steroid-associated osteonecrosis in rats

	A novel bone targeting delivery system carrying phytomolecule icaritin for prevention of steroid-associated osteonecrosis in rats
	Shihui Chen; Lizhen Zheng; Jiayong Zhang; Heng Wu; Nan Wang; Wenxue Tong; Jiankun Xu; Le Huang; Yifeng Zhang; Zhijun Yang
刊名	Bone
	2018
文献子类	期刊论文
英文摘要	One of the effective strategies for prevention of steroid-associated osteonecrosis (SAON) is to inhibit bone resorption and fat formation and promote bone formation at osteonecrotic sensitive skeletal sites. We identified a novel phytomolecule that showed positive effects on osteogenesis, anti-bone resorption and anti-adipogenesis in vitro and also developed a bonetargeting delivery system (BTDS) for in vivo experimental study. The study investigated if our innovative synthesized BTDS carrying this phytomolecule would be able to effectively prevent SAON in a rat model. SAON was induced by combined injections of lipopolysaccharide and methylprednisolone. SAON rats were divided into four groups, one SAON untreated control group and three SAON treatment groups with different types of delivery systems (Asp8-liposome-icaritin, liposome-icaritin and Asp8-liposome) for two weeks. SAON lesions were identified and osteoclasts activity, osteogenesis and adipogenesis at these sites were evaluated by immunohistochemistry. Ex vitro study was also designed to evaluate the osteogenic and adipogenic potential of the isolated bone marrow stromal cells (BMSCs) via real-time PCR and histochemical staining. Our results showed that as a bone surface-specific BTDS, Asp8-liposome-icaritin effectively prevented steroidstreated rats from SAON with significantly decreased osteocytes apoptosis, down-regulated osteoclatsogenesis and up-regulated osteogenesis. However, both liposome-icaritin and Asp8- liposome treatment did not show significant efficacy for SAON prevention. In summary, this proof-concept-study showed for the first time that the innovative Asp8-liposome-icaritin BTDS was effective for prevention of SAON in terms of bone resorption prevention, adipogenesis suppression, and bone-formation enhancement.
URL标识	查看原文
语种	英语
WOS记录号	WOS:000417552200008
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/12154]
专题	深圳先进技术研究院_医工所
作者单位	Bone
推荐引用方式 GB/T 7714	Shihui Chen,Lizhen Zheng,Jiayong Zhang,et al. A novel bone targeting delivery system carrying phytomolecule icaritin for prevention of steroid-associated osteonecrosis in rats[J]. Bone,2018.
APA	Shihui Chen.,Lizhen Zheng.,Jiayong Zhang.,Heng Wu.,Nan Wang.,...&Ling Qin.(2018).A novel bone targeting delivery system carrying phytomolecule icaritin for prevention of steroid-associated osteonecrosis in rats.Bone.
MLA	Shihui Chen,et al."A novel bone targeting delivery system carrying phytomolecule icaritin for prevention of steroid-associated osteonecrosis in rats".Bone (2018).