An integrated microfluidic device for rapid and high-sensitivity analysis of circulating tumor cells

	An integrated microfluidic device for rapid and high-sensitivity analysis of circulating tumor cells
	Jianing Jiang; Hui Zhao; Weiliang Shu; Jing Tian; Yuqing Huang; Yongxin Song; Ruoyu Wang; Encheng Li; Dennis Slamon; Dongmei Hou
刊名	SCIENTIFIC REPORTS
	2017
文献子类	期刊论文
英文摘要	Recently there has been a more focus on the development of an efficient technique for detection of circulating tumor cells (CTCs), due to their significance in prognosis and therapy of metastatic cancer. However, it remains a challenge because of the low count of CTCs in the blood. Herein, a rapid and high-sensitivity approach for CTCs detection using an integrated microfluidic system, consisting of a deterministic lateral displacement (DLD) isolating structure, an automatic purifying device with CD45-labeled immunomagnetic beads and a capturing platform coated with rat-tail collagen was reported. We observed high capture rate of 90%, purity of about 50% and viability of more than 90% at the high throughput of 1 mL/min by capturing green fluorescent protein (GFP)-positive cells from blood. Further capturing of CTCs from metastatic cancers patients revealed a positive capture rate of 83.3%. Furthermore, our device was compared with CellSearch system via parallel analysis of 30 cancer patients, to find no significant difference between the capture efficiency of both methods. However, our device displayed advantage in terms of time, sample volume and cost for analysis. Thus, our integrated device with sterile environment and convenient use will be a promising platform for CTCs detection with potential clinical application.
URL标识	查看原文
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/12019]
专题	深圳先进技术研究院_医工所
作者单位	SCIENTIFIC REPORTS
推荐引用方式 GB/T 7714	Jianing Jiang,Hui Zhao,Weiliang Shu,et al. An integrated microfluidic device for rapid and high-sensitivity analysis of circulating tumor cells[J]. SCIENTIFIC REPORTS,2017.
APA	Jianing Jiang.,Hui Zhao.,Weiliang Shu.,Jing Tian.,Yuqing Huang.,...&Lichuan Zhang.(2017).An integrated microfluidic device for rapid and high-sensitivity analysis of circulating tumor cells.SCIENTIFIC REPORTS.
MLA	Jianing Jiang,et al."An integrated microfluidic device for rapid and high-sensitivity analysis of circulating tumor cells".SCIENTIFIC REPORTS (2017).