New design for multi-crystal data collection at SSRF

doi:10.1007/s41365-018-0357-5

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	New design for multi-crystal data collection at SSRF
	Li, B ; Huang, S ; Pan, QY ; Li, MJ ; Zhou, H ; Wang, QS ; Yu, F ; Sun, B ; Chen, JQ ; He, JH
刊名	NUCLEAR SCIENCE AND TECHNIQUES
	2018
卷号	29 期号:2 页码:-
关键词	Membrane-proteins Crystallography Radiation Chip
ISSN号	1001-8042
DOI	10.1007/s41365-018-0357-5
文献子类	期刊论文
英文摘要	Data collection with microcrystals at synchrotron radiation facilities is challenging because it is difficult to harvest and locate microcrystals. Moreover, microcrystals are sensitive to radiation damage; thus, typically, a complete data set cannot be obtained with a single microcrystal. Herein, we report a new method for data collection with multiple microcrystals having a crystal size ranging from 1 to 30 mu m. This method is suitable for not only low-temperature (100 K) data collection but also room-temperature data collection. Thin Kapton membranes were used to capture multiple crystals simultaneously. The microcrystals were visible under an optical microscope and easily located because the membrane was transparent and sufficiently thin. The film was fixed to a bracket that was prepared using a three-dimensional printer. The bracket was fixed on a magnetic base via screwing and employed by the goniometer system for data collection. Multiple data sets of fatty acid-binding protein 4 (FABP4) and lysozyme microcrystals were collected using this novel designed device. Finally, the structures of protein FABP4 and lysozyme were obtained from these data via the molecule replacement method. The data statistics reveal that this method provides a comparable result to traditional methods such as a nylon loop.
语种	英语
WOS记录号	WOS:000427326900005
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/29084]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.Li, B 2.Huang, S 3.Pan, QY 4.Li, MJ 5.Zhou, H 6.Wang, QS 7.Yu, F 8.Sun, B 9.Chen, JQ 10.He, JH
推荐引用方式 GB/T 7714	Li, B,Huang, S,Pan, QY,et al. New design for multi-crystal data collection at SSRF[J]. NUCLEAR SCIENCE AND TECHNIQUES,2018,29(2):-.
APA	Li, B.,Huang, S.,Pan, QY.,Li, MJ.,Zhou, H.,...&He, JH.(2018).New design for multi-crystal data collection at SSRF.NUCLEAR SCIENCE AND TECHNIQUES,29(2),-.
MLA	Li, B,et al."New design for multi-crystal data collection at SSRF".NUCLEAR SCIENCE AND TECHNIQUES 29.2(2018):-.