Bacterial Analysis Using an Electrochemical DNA Biosensor with Poly-Adenine-Mediated DNA Self-Assembly

doi:10.1021/acsami.7b17327

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Bacterial Analysis Using an Electrochemical DNA Biosensor with Poly-Adenine-Mediated DNA Self-Assembly
	Li, LY ; Wang, LL ; Xu, Q ; Xu, L ; Liang, W ; Li, Y ; Ding, M ; Aldalbahi, A ; Ge, ZL ; Wang, LH
刊名	ACS APPLIED MATERIALS & INTERFACES
	2018
卷号	10 期号:8 页码:6895-6903
关键词	Sequence-specific Detection Incorporated Nonfouling Surface Single-nucleotide Polymorphism Gold Nanoparticles Label-free Escherichia-coli Pcr Amplicons Stranded-dna Nanostructure Sensor
ISSN号	1944-8244
DOI	10.1021/acsami.7b17327
文献子类	期刊论文
英文摘要	The spatial arrangement of DNA probes on the electrode surface is of critical significance for the performance probe surface remains challenging. In this work, we develop a of electrochemical biosensors. However, rational control of the capture probe carrying a poly-adenine anchoring block to construct a programmable self-assembled monolayer for a "sandwich-type" electrochemical biosensor. We show that with a co-assembling strategy using a polyA capture probe and 6-mercapto-l-hexanol, the density of the probes on the gold electrode can be simply adjusted by the length of polyA. The electron-transfer effect and thus the hybridization efficiency can as well be optimized by tuning the polyA length. As a result, we obtained an excellent biosensor performance with a limit of detection as low as 5 fM for a synthetic DNA target. We demonstrate the practicability of this system by analyzing a PCR product from Escherichia coli genomic DNA (0.2 pg/mu L). On the basis of the ideal electrochemical interface, our polyA-based biosensor exhibited excellent reusability and stability, which is important for potential applications in the onsite analysis for a wide range of targets.
语种	英语
WOS记录号	WOS:000426618000015
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/28933]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.Li, LY 2.Wang, LL 3.Xu, Q 4.Xu, L 5.Liang, W 6.Li, Y 7.Ding, M 8.Aldalbahi, A 9.Ge, ZL 10.Wang, LH
推荐引用方式 GB/T 7714	Li, LY,Wang, LL,Xu, Q,et al. Bacterial Analysis Using an Electrochemical DNA Biosensor with Poly-Adenine-Mediated DNA Self-Assembly[J]. ACS APPLIED MATERIALS & INTERFACES,2018,10(8):6895-6903.
APA	Li, LY.,Wang, LL.,Xu, Q.,Xu, L.,Liang, W.,...&Liu, G.(2018).Bacterial Analysis Using an Electrochemical DNA Biosensor with Poly-Adenine-Mediated DNA Self-Assembly.ACS APPLIED MATERIALS & INTERFACES,10(8),6895-6903.
MLA	Li, LY,et al."Bacterial Analysis Using an Electrochemical DNA Biosensor with Poly-Adenine-Mediated DNA Self-Assembly".ACS APPLIED MATERIALS & INTERFACES 10.8(2018):6895-6903.