Nuclease-free target recycling signal amplification for ultrasensitive multiplexing DNA biosensing

doi:10.1016/j.bios.2017.03.051

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Nuclease-free target recycling signal amplification for ultrasensitive multiplexing DNA biosensing
	Zhao, ZH; Chen, SX; Wang, JB; Su, J; Xu, JQ; Mathur, S; Fan, CH; Song, SP
刊名	BIOSENSORS & BIOELECTRONICS
	2017
卷号	94 期号:-页码:605-608
关键词	Label-free Chemiluminescence Detection Strategy Acid
ISSN号	0956-5663
DOI	10.1016/j.bios.2017.03.051
文献子类	期刊论文
英文摘要	Ultrasensitive biosensing technologies without gene amplification held great promise for direct detection of DNA. Herein we report a novel biosensing method, combining target recycling signal-amplification strategy and a homemade electrochemical device. Especially, the target recycling was achieved by a strand displacement process, no needing the help of any nucleases. In the presence of target DNA, the recycling system could be activated to generate a cascade of assembly steps with three hairpin DNA segments. Each recycling process were accompanied by a disassembly step that the last hairpin DNA segment displaces target DNA from the complex at the end of each circulation, freeing targets to activate the self-assembly of more trefoil DNA structures. This biosensing method could detect target DNA at aM level and can distinguish target DNA from interfering DNAs, demonstrating its high sensitivity and high selectivity. Importantly, the biosensing method could work well with serum samples.
WOS关键词	LABEL-FREE ; CHEMILUMINESCENCE DETECTION ; STRATEGY ; ACID
语种	英语
WOS记录号	WOS:000401201300079
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/28754]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
推荐引用方式 GB/T 7714	Zhao, ZH,Chen, SX,Wang, JB,et al. Nuclease-free target recycling signal amplification for ultrasensitive multiplexing DNA biosensing[J]. BIOSENSORS & BIOELECTRONICS,2017,94(-):605-608.
APA	Zhao, ZH.,Chen, SX.,Wang, JB.,Su, J.,Xu, JQ.,...&Song, SP.(2017).Nuclease-free target recycling signal amplification for ultrasensitive multiplexing DNA biosensing.BIOSENSORS & BIOELECTRONICS,94(-),605-608.
MLA	Zhao, ZH,et al."Nuclease-free target recycling signal amplification for ultrasensitive multiplexing DNA biosensing".BIOSENSORS & BIOELECTRONICS 94.-(2017):605-608.