DNA Nanostructure-Based Engineering of the Biosensing Interface for Biomolecular Detection

doi:10.7536/PC161214

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	DNA Nanostructure-Based Engineering of the Biosensing Interface for Biomolecular Detection
	Ye, DK; Zuo, XL; Fan, CH
刊名	PROGRESS IN CHEMISTRY
	2017
卷号	29 期号:1 页码:36-46
关键词	Biosensing Interfacial Engineering Dna Nanostructure Electrochemical Cell Imaging
ISSN号	1005-281X
DOI	10.7536/PC161214
文献子类	期刊论文
英文摘要	The biosensing technology plays an important role in environmental monitoring, safety control and medical diagnosis. Precise control of the interaction between bio-recognition probe and the interface is critical to improve the sensitivity, specificity and selectivity of biosensors. In a typical bioprobe immobilization, the heterogeneity of self-assembled monolayers on the surface increases the binding energy barrier and decreases the recognition efficiency and rate. We found that DNA nanostructures, such as tetrahedral DNA nanostructures (TDNs), could increase the homogeneity of self-assembled monolayers via enthalpy-entropy compensation, which enables precise regulation of interfacial property at the nanoscale. By regulating the intermolecular distance of bioprobes, the hybridization efficiency and hybridization rate of DNA probes can be improved significantly. The detection limit of DNA and microRNA can be pushed down to 10 aM limit. The detection limit of antigen detection can be improved to 100 pM and the detection limit of small molecule (cocaine) can be pushed to 33 nM. By using TDNs, we developed a universal detection platform for nucleic acids, proteins, small molecules and cells with superior detection sensitivity. To further use TDN probes in cells and in vivo, we explored the transport pathways of TDNs into the cell and directed their targeting location to specific organelles. We aim to develop DNA nanostructure-based bioprobes for intracellular and in-vivo imaging.
语种	英语
WOS记录号	WOS:000395960100012
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/27299]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
推荐引用方式 GB/T 7714	Ye, DK,Zuo, XL,Fan, CH. DNA Nanostructure-Based Engineering of the Biosensing Interface for Biomolecular Detection[J]. PROGRESS IN CHEMISTRY,2017,29(1):36-46.
APA	Ye, DK,Zuo, XL,&Fan, CH.(2017).DNA Nanostructure-Based Engineering of the Biosensing Interface for Biomolecular Detection.PROGRESS IN CHEMISTRY,29(1),36-46.
MLA	Ye, DK,et al."DNA Nanostructure-Based Engineering of the Biosensing Interface for Biomolecular Detection".PROGRESS IN CHEMISTRY 29.1(2017):36-46.