Probing the Dynamic Interaction between Damaged DNA and a Cellular Responsive Protein Using a Piezoelectric Mass Biosensor

	Probing the Dynamic Interaction between Damaged DNA and a Cellular Responsive Protein Using a Piezoelectric Mass Biosensor
	Jin, Yulong 1,2; Xie, Yunfeng 1,2,3; Wu, Kui 1,2; Huang, Yanyan 1,2; Wang, Fuyi 1,2; Zhao, Rui 1,2
刊名	ACS APPLIED MATERIALS & INTERFACES
	2017-03-15
卷号	9 期号:10 页码:8490-8497
关键词	Dna Damage Piezoelectric Mass Biosensor High-mobility-group Box 1 Molecular Recognition Kinetic Analysis
英文摘要	The binding events between damaged DNA and recognition biomolecules are of great interest for understanding the activity of DNA-damaging drugs and the related DNA repair networks. Herein, a simple and sensitive sensor system was tailored for real-time probing of the dynamic molecular recognition between cisplatin-damaged-DNA (cisPt-DNA) and a cellular responsive protein, high mobility-group box 1 (HMGB1). By integration of flow injection analysis (FIA) with quartz crystal microbalance (QCM), the interaction time-course of cisPt-DNA and HMGB1 domain A (HMGB1a) was investigated. The highly specific sensing interface was carefully designed and fabricated using cisPt-DNA as recognition element. A hybrid self-assembled monolayer consisting of cysteamine and mercaptohexanol was introduced to resist nonspecific adsorption. The calculated kinetic parameters (k(ass) and k(diss)) and the dissociation constant (KD) demonstrated the rapid recognition and tight binding of HMGBla toward cisPt-DNA. Molecular docking was employed to simulate the complex formed by cisPt-DNA and HMGB1a. The tight binding of such a DNA-damage responsive complex is appealing for the downstream molecular recognition event related to the resistance to DNA repair. This continuous-flow QCM biosensor is an ideal tool for studying specific interactions between drug-damaged-DNAs and their recognition proteins in a physiological-relevant environment, and will provide a potential sensor platform for rapid screening and evaluating metal anticancer drugs.
语种	英语
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/38257]
专题	化学研究所_分析测试中心
作者单位	1.Chinese Acad Sci, Inst Chem, CAS Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.China Natl Cereals Oils & Foodstuffs Corp, Beijing 100020, Peoples R China
推荐引用方式 GB/T 7714	Jin, Yulong,Xie, Yunfeng,Wu, Kui,et al. Probing the Dynamic Interaction between Damaged DNA and a Cellular Responsive Protein Using a Piezoelectric Mass Biosensor[J]. ACS APPLIED MATERIALS & INTERFACES,2017,9(10):8490-8497.
APA	Jin, Yulong,Xie, Yunfeng,Wu, Kui,Huang, Yanyan,Wang, Fuyi,&Zhao, Rui.(2017).Probing the Dynamic Interaction between Damaged DNA and a Cellular Responsive Protein Using a Piezoelectric Mass Biosensor.ACS APPLIED MATERIALS & INTERFACES,9(10),8490-8497.
MLA	Jin, Yulong,et al."Probing the Dynamic Interaction between Damaged DNA and a Cellular Responsive Protein Using a Piezoelectric Mass Biosensor".ACS APPLIED MATERIALS & INTERFACES 9.10(2017):8490-8497.