Enantioselectivity of D-amino acid oxidase in the presence of ionic liquids | |
Liu, Qingju1; Zhao, Chuanfang1; Huang, Jincheng1; Chen, Li1; Yang, Kunhao1; Gong, Lingling1; Du, Yuguo1; Yu, Chuyi3; Wu, Li1; Li, Xiangjun1 | |
刊名 | RSC ADVANCES |
2017 | |
卷号 | 7期号:54页码:34110-34117 |
英文摘要 | In this paper, enantioselectivities of D-amino acid oxidase (DAAO) in ten ionic liquids were investigated in detail. Among the ionic liquids studied, 3-methylimidazolium formate ([MIM][COO]) was able to trigger enzyme activation with L-Ala as substrate. The enzyme activity of DAAO reached a maximum of 4.26 U mg(-1) min(-1) in the presence of 40% [MIM][COO], while no activity toward L-Ala was observed when DAAO was incubated with other ILs. Moreover, DAAO activities towards other L-amino acids such as L-Pro and L-Arg were also improved in [MIM][COO], which indicated that the enzymatic enantioselectivity of amino acids was lowered. This was further confirmed by capillary electrophoresis, circular dichrosim and fluorescence analysis. Molecular optimization supported that the protein-ionic liquid interactions modulated the structure of the enzyme, especially in [MIM][COO], leading to relaxation in the enantioselectivity of DAAO. This observation and exploration might open a new window for modulation enantioselectivity resolution in ionic liquids. |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.iccas.ac.cn/handle/121111/39068] |
专题 | 化学研究所_分子识别与功能实验室 |
作者单位 | 1.Univ Chinese Acad Sci, Sch Chem & Chem Engn, Beijing 101408, Peoples R China 2.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China 3.Chinese Acad Sci, Lab Mol Recognit & Select Synth, Inst Chem, Beijing 100191, Peoples R China |
推荐引用方式 GB/T 7714 | Liu, Qingju,Zhao, Chuanfang,Huang, Jincheng,et al. Enantioselectivity of D-amino acid oxidase in the presence of ionic liquids[J]. RSC ADVANCES,2017,7(54):34110-34117. |
APA | Liu, Qingju.,Zhao, Chuanfang.,Huang, Jincheng.,Chen, Li.,Yang, Kunhao.,...&He, Yujian.(2017).Enantioselectivity of D-amino acid oxidase in the presence of ionic liquids.RSC ADVANCES,7(54),34110-34117. |
MLA | Liu, Qingju,et al."Enantioselectivity of D-amino acid oxidase in the presence of ionic liquids".RSC ADVANCES 7.54(2017):34110-34117. |
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