Chlorhexidine-Loaded Amorphous Calcium Phosphate Nanoparticles for Inhibiting Degradation and Inducing Mineralization of Type I Collagen

	Chlorhexidine-Loaded Amorphous Calcium Phosphate Nanoparticles for Inhibiting Degradation and Inducing Mineralization of Type I Collagen
	Cai, Xue 1; Han, Bing 1; Liu, Yan 2; Tian, Fucong 1; Liang, Fuxin 3; Wang, Xiaoyan 1
刊名	ACS APPLIED MATERIALS & INTERFACES
	2017-04-19
卷号	9 期号:15 页码:12949-12958
关键词	Amorphous Calcium Phosphate Chlorhexidine Sustained Release Nanoparticle Mineralization Type i Collagen
英文摘要	A major shortcoming of contemporary dentin adhesives is their limited durability. Exposed collagen fibrils Within the bonding interface are degraded by matrix metalloproteinases (MMPs), resulting in aging of the resin dentin bond. In this study, chlorhexidine-loaded amorphous calcium phosphate (ACP) nanoparticies were synthesized to induce the mineralization of collagen fibrils. The nanoparticles sustainably released chlorhexidine to inhibit MMPs during mineralization. Three types of ACP nanoparticies were prepared: N-ACP containing no chlorhexidine, C-ACP containing chlorhexidine acetate, and G-ACP containing chlorhexidine gluconate, whidi had a higher drug-loading than CACP: Scanning and transmission electron microscopy indicated that the synthesized nanoparticles had diameters of less than 100 nm. Some had diameters of less than 40 nm, which was smaller than the width of gap zones in the collagen fibrils. Energy dispersive X-ray spectroscopy, Fourier-transform infrared spectrokopy, and high performance liquid chromatography confirmed the presence of chlorhexidine in the nanoparticies. X-ray diffraction confirmed that the nanoparticles were amorphous. The drug. loading was 0.11% for C-ACP and 0.53% for G-ACP. In vitro release profiles indicated that chlorhexidine was released sustainably via first-order kinetics. Released chlorhexidine inhibited the degradation of collagen in human dentine powder, and its effect lasted longer thari,that of pure chlothexidine of the same concentration. The ACP could induce the mineralization of self assembled type I collagen fibrils. The chlorhexidine-loaded ACP nanoparticies sustainably released chlorhexidine and ACP under appropriate conditions. This is useful for inhibiting degradation and inducing the mineralization of dentine collagen fibrils.
语种	英语
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/39024]
专题	化学研究所_高分子物理与化学实验室
作者单位	1.Peking Univ, Sch & Hosp Stomatol, Beijing Key Lab Digital Stomatol, Natl Engn Lab Digital & Mat Technol Stomatol,Dept, Beijing 100081, Peoples R China 2.Peking Univ, Sch & Hosp Stomatol, Beijing Key Lab Digital Stomatol, Dept Orthodont,Natl Engn Lab Digital & Mat Techno, Beijing 100081, Peoples R China 3.Chinese Acad Sci, Inst Chem, State Key Lab Polymer Phys & Chem, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Cai, Xue,Han, Bing,Liu, Yan,et al. Chlorhexidine-Loaded Amorphous Calcium Phosphate Nanoparticles for Inhibiting Degradation and Inducing Mineralization of Type I Collagen[J]. ACS APPLIED MATERIALS & INTERFACES,2017,9(15):12949-12958.
APA	Cai, Xue,Han, Bing,Liu, Yan,Tian, Fucong,Liang, Fuxin,&Wang, Xiaoyan.(2017).Chlorhexidine-Loaded Amorphous Calcium Phosphate Nanoparticles for Inhibiting Degradation and Inducing Mineralization of Type I Collagen.ACS APPLIED MATERIALS & INTERFACES,9(15),12949-12958.
MLA	Cai, Xue,et al."Chlorhexidine-Loaded Amorphous Calcium Phosphate Nanoparticles for Inhibiting Degradation and Inducing Mineralization of Type I Collagen".ACS APPLIED MATERIALS & INTERFACES 9.15(2017):12949-12958.