Sensitive determination of endogenous hydroxyl radical in live cell by a BODIPY based fluorescent probe

CORC > 化学研究所 > 中国科学院化学研究所 > 分子动态与稳态结构实验室

	Sensitive determination of endogenous hydroxyl radical in live cell by a BODIPY based fluorescent probe
	Lei, Kepeng 1,2; Sun, Mingtai 1; Du, Libo 5; Zhang, Xiaojie 5; Yu, Huan 1; Wang, Suhua 1,2,3,4; Hayat, Tasawar 3; Alsaedi, Ahmed 3
刊名	TALANTA
	2017-08-01
卷号	170 页码:314-321
关键词	Bodipy Cell Imaging Fluorescent Probe Hydroxyl Radical Sensitive And Selective
英文摘要	The sensitive and selective fluorescence probe for hydroxyl radical analysis is of significance because hydroxyl radical plays key roles in many physiological and pathological processes. In this work, a novel organic fluorescence molecular probe OHP for hydroxyl radical is synthesized by a two-step route. The probe employs 4-bora-3a,4a-diaza-s-indacene (difluoroboron dipyrromethene, BODIPY) as the fluorophore and possesses relatively high fluorescence quantum yields (77.14%). Hydroxyl radical can rapidly react with the probe and quench the fluorescence in a good linear relationship (R-2=0.9967). The limit of detection is determined to be as low as 11 nM. In addition, it has been demonstrated that the probe has a good stability against pH and light illumination, low cytotoxicity and high biocompatibility. Cell culture experimental results show that the probe OHP is sensitive and selective for imaging and tracking endogenous hydroxyl radical in live cells.
语种	英语
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/38551]
专题	化学研究所_分子动态与稳态结构实验室
作者单位	1.Chinese Acad Sci, Inst Intelligent Machines, Hefei 230031, Anhui, Peoples R China 2.Univ Sci & Technol China, Dept Mat Sci & Engn, Hefei 230026, Anhui, Peoples R China 3.King Abdulaziz Univ, NAAM Res Grp, Fac Sci, Jeddah 21589, Saudi Arabia 4.North China Elect Power Univ, Sch Environm & Chem Engn, Beijing 102206, Peoples R China 5.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, State Key Lab Struct Chem Unstable & Stable Speci, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Lei, Kepeng,Sun, Mingtai,Du, Libo,et al. Sensitive determination of endogenous hydroxyl radical in live cell by a BODIPY based fluorescent probe[J]. TALANTA,2017,170:314-321.
APA	Lei, Kepeng.,Sun, Mingtai.,Du, Libo.,Zhang, Xiaojie.,Yu, Huan.,...&Alsaedi, Ahmed.(2017).Sensitive determination of endogenous hydroxyl radical in live cell by a BODIPY based fluorescent probe.TALANTA,170,314-321.
MLA	Lei, Kepeng,et al."Sensitive determination of endogenous hydroxyl radical in live cell by a BODIPY based fluorescent probe".TALANTA 170(2017):314-321.