Nanoscale Distribution of Transforming Growth Factor Receptor on Post-Golgi Vesicle Revealed by Super-resolution Microscopy

CORC > 化学研究所 > 中国科学院化学研究所 > 分子纳米结构与纳米技术实验室

	Nanoscale Distribution of Transforming Growth Factor Receptor on Post-Golgi Vesicle Revealed by Super-resolution Microscopy
	Ruan, Hefei 1,2; Yu, Jianqiang 1; Yuan, Jinghe 1; Li, Nan 1,2; Fang, Xiaohong 1,2
刊名	CHEMISTRY-AN ASIAN JOURNAL
	2016-12-06
卷号	11 期号:23 页码:3359-3364
关键词	Clusters Receptors Stimulated Emission Depletion Microscopy Super-resolution Microscopy Vesicles
英文摘要	Transforming growth factor-beta (TGF-beta) type II receptor (T beta RII) plays a critical role in the initiation of TGF-beta signaling pathway; therefore, the study of its synthesis and transport processes is of great important. In this work, we achieved super-resolution imaging of a new type of T beta RII-containing post-Golgi vesicle by our home-built stimulated emission depletion (STED) microscope. We visualized the ring-shaped structure of these vesicles containing newly synthesized T beta RII in the cytoplasm and characterized their size distribution from 300 to 1000 nm. These vesicles could be swollen by chloroquine treatment. Further investigation revealed that T beta RII formed clusters on the outer ring of the post-Golgi vesicles. This study offers new information on the intracellular transportation of TGF-beta receptors for better understanding its signaling process.
语种	英语
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/38037]
专题	化学研究所_分子纳米结构与纳米技术实验室
作者单位	1.Chinese Acad Sci, Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing Natl Lab Mol Sci, 2 ZhongGuanCun North First St, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Ruan, Hefei,Yu, Jianqiang,Yuan, Jinghe,et al. Nanoscale Distribution of Transforming Growth Factor Receptor on Post-Golgi Vesicle Revealed by Super-resolution Microscopy[J]. CHEMISTRY-AN ASIAN JOURNAL,2016,11(23):3359-3364.
APA	Ruan, Hefei,Yu, Jianqiang,Yuan, Jinghe,Li, Nan,&Fang, Xiaohong.(2016).Nanoscale Distribution of Transforming Growth Factor Receptor on Post-Golgi Vesicle Revealed by Super-resolution Microscopy.CHEMISTRY-AN ASIAN JOURNAL,11(23),3359-3364.
MLA	Ruan, Hefei,et al."Nanoscale Distribution of Transforming Growth Factor Receptor on Post-Golgi Vesicle Revealed by Super-resolution Microscopy".CHEMISTRY-AN ASIAN JOURNAL 11.23(2016):3359-3364.