Isolation and characterization of 11 microsatellite loci from Camellia sinensis in Taiwan using PCR-based isolation of microsatellite arrays (PIMA)

doi:10.1007/s10592-007-9391-2

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 资源植物与生物技术所级重点实验室

	Isolation and characterization of 11 microsatellite loci from Camellia sinensis in Taiwan using PCR-based isolation of microsatellite arrays (PIMA)
	Hung, Cheng-Yu 1; Wang, Kuo-Hsiung 1; Huang, Chi-Chun 1; Gong, Xun2 ; Ge, Xue-Jun 3; Chiang, Tzen-Yuh 1
刊名	CONSERVATION GENETICS
	2008-06-01
卷号	9 期号:3 页码:779-781
关键词	Camellia Sinensis Heterozygosity Microsatellite Fingerprinting Pima Rapd-pcr Enrichment
ISSN号	1566-0621
DOI	10.1007/s10592-007-9391-2
通讯作者	tychiang@mail.ncku.edu.tw
文献子类	Article
英文摘要	We report 11 novel microsatellite primer pairs for the wild tea, Camellia sinensis (L.) O. Kuntze forma formosensis Kitamura. These simple sequence repeat markers were tested in 24 samples collected from wild tea populations, and in cultivars and C. japonica. The number of alleles ranged from 4 to18. The expected (H(E)) and observed (H(O)) heterozygosity were 0.687-0.946 and 0.042-0.792, respectively. All loci were significantly deviated from Hardy-Weinberg expectations due to the heterozygote deficiency, indicating a dramatic loss of genetic polymorphisms in the rare species. Significant LD was discovered in most loci. These primers may provide a tool for understanding demography and population structure in wild tea.
学科主题	Biodiversity Conservation ; Genetics & Heredity
WOS关键词	TEA ; CLONES
WOS研究方向	Biodiversity & Conservation ; Genetics & Heredity
语种	英语
WOS记录号	WOS:000255191000038
公开日期	2011-12-06
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn:8080/handle/151853/2515]
专题	昆明植物研究所_资源植物与生物技术所级重点实验室
作者单位	1.Natl Cheng Kung Univ, Dept Life Sci, Tainan 701, Taiwan 2.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China 3.Sun Yat Sen Univ, State Key Lab Biocontrol Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Hung, Cheng-Yu,Wang, Kuo-Hsiung,Huang, Chi-Chun,et al. Isolation and characterization of 11 microsatellite loci from Camellia sinensis in Taiwan using PCR-based isolation of microsatellite arrays (PIMA)[J]. CONSERVATION GENETICS,2008,9(3):779-781.
APA	Hung, Cheng-Yu,Wang, Kuo-Hsiung,Huang, Chi-Chun,Gong, Xun,Ge, Xue-Jun,&Chiang, Tzen-Yuh.(2008).Isolation and characterization of 11 microsatellite loci from Camellia sinensis in Taiwan using PCR-based isolation of microsatellite arrays (PIMA).CONSERVATION GENETICS,9(3),779-781.
MLA	Hung, Cheng-Yu,et al."Isolation and characterization of 11 microsatellite loci from Camellia sinensis in Taiwan using PCR-based isolation of microsatellite arrays (PIMA)".CONSERVATION GENETICS 9.3(2008):779-781.