Detection of Valeriana jatamansi as an Adulterant of Medicinal Paris by Length Variation of Chloroplast psbA-trnH Region

doi:10.1055/s-0030-1250072

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 中国科学院东亚植物多样性与生物地理学重点实验室

	Detection of Valeriana jatamansi as an Adulterant of Medicinal Paris by Length Variation of Chloroplast psbA-trnH Region
	Yang, Ying 1,2; Zhai, Yanhong 1,2; Liu, Tao 3; Zhang, Fangming 1,2; Ji, Yunheng1
刊名	PLANTA MEDICA
	2011
卷号	77 期号:1 页码:87-91
关键词	Paris Polyphylla Var. Chinensis Paris Polyphylla Var. Yunnanensis Melanthiaceae Valeriana Jatamansi Valerianaceae Molecular Authentication Psba-trnh
ISSN号	0032-0943
DOI	10.1055/s-0030-1250072
通讯作者	jiyh@mail.kib.ac.cn
文献子类	Article
英文摘要	Two varieties of Paris polyphylla Smith (Melanthiaceae), P. polyphylla Smith var. chinensis (Franch.) Hara, and P. polyphylla Smith var. yunnanensis (Franch.) Hand.-Mazz., are used as medicinal Paris in China. Their dried rhizomes are the major source of raw material for some medicines. In recent years, medicinal Paris has been found to be adulterated with Valeriana jatamansi Jones (Valerianaceae) due to its high market demand and natural resource deficiency. After the chloroplast psbA-trnH regions of medicinal Paris and V. jatamansi were sequenced and analyzed, it was found that their characteristic sizes were > 1000 and around 250 bp, respectively. Based on length variation, medicinal Paris and the mixed adulterant were detected and distinguished from each other by amplification and electrophoresis. The amount of V. jatamansi that can be identified as an adulterant of medicinal Paris was also investigated. A trace amount (1 : 1000) of the adulterant was detected in the sensitivity tests. The established method has been proven to be sensitive and reliable.
学科主题	Plant Sciences ; Chemistry, Medicinal ; Pharmacology & Pharmacy
WOS关键词	DNA BARCODES ; PHYLOGENY ; BIOGEOGRAPHY ; AUTHENTICATION ; DIPSACALES ; PLANTS ; PCR
WOS研究方向	Plant Sciences ; Pharmacology & Pharmacy
语种	英语
WOS记录号	WOS:000285406500018
资助机构	Natural Science Foundation of China (NSFC)[30670132]; Natural Science Foundation of Yunnan Province (NSFN)[2007C089M]; State Key Laboratory of Phytochemistry and Plant Resources in West China; Germplasm Bank of Wild Species; CAS of the Kunming Institute of Botany
公开日期	2012-03-16
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn:8080/handle/151853/3896]
专题	昆明植物研究所_中国科学院东亚植物多样性与生物地理学重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Biodivers & Biogeog, Kunming Inst Bot, Kunming 650204, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 3.Yunnan Agr Univ, Fac Agron & Biotechnol, Kunming, Peoples R China
推荐引用方式 GB/T 7714	Yang, Ying,Zhai, Yanhong,Liu, Tao,et al. Detection of Valeriana jatamansi as an Adulterant of Medicinal Paris by Length Variation of Chloroplast psbA-trnH Region[J]. PLANTA MEDICA,2011,77(1):87-91.
APA	Yang, Ying,Zhai, Yanhong,Liu, Tao,Zhang, Fangming,&Ji, Yunheng.(2011).Detection of Valeriana jatamansi as an Adulterant of Medicinal Paris by Length Variation of Chloroplast psbA-trnH Region.PLANTA MEDICA,77(1),87-91.
MLA	Yang, Ying,et al."Detection of Valeriana jatamansi as an Adulterant of Medicinal Paris by Length Variation of Chloroplast psbA-trnH Region".PLANTA MEDICA 77.1(2011):87-91.