Proteomic profiling and redox status alteration of recalcitrant tea (Camellia sinensis) seed in response to desiccation

doi:10.1007/s00425-010-1322-7

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 中国科学院东亚植物多样性与生物地理学重点实验室

	Proteomic profiling and redox status alteration of recalcitrant tea (Camellia sinensis) seed in response to desiccation
	Chen, Qi 1,2; Yang, Liming 1,3; Ahmad, Parvaiz 4; Wan, Xiaochun 2; Hu, Xiangyang 1
刊名	PLANTA
	2011-03-01
卷号	233 期号:3 页码:583-592
关键词	Camellia Desiccation Proteomic Reactive Oxygen Species Recalcitrant Seeds
ISSN号	0032-0935
DOI	10.1007/s00425-010-1322-7
通讯作者	Huxiangyang@mail.kib.ac.cn
文献子类	Article
英文摘要	Tea seed is believed to be recalcitrant based on its sensitivity to chilling or drying stress. Reactive oxygen species (ROS) and alterations in cytosolic redox status have been implicated in intolerance to desiccation by recalcitrant seed, but there is little information available regarding how ROS are regulated in seeds susceptible to drying stress. We investigated changes in protein expression and activity in tea embryo in response to desiccation using physiological and proteomic methods. Results showed that desiccation treatment dramatically induced the accumulation of H2O2 in tea embryos, accompanied by increased activities of antioxidant enzymes like ascorbate peroxidase (APX) and superoxide dismutase (SOD). Proteomic analyses also demonstrated that 23 proteins associated with defense response, metabolism and redox status were up-regulated following desiccation. Increase in antioxidants, ascorbic acid (AsA) and catalase (CAT) (H2O2 scavengers) partially assuaged desiccation damage to tea seed, resulting in improved germination rates. Higher accumulation of H2O2 aggravated desiccation damage to seeds leading to lower germination activity. We propose that desiccation causes an over-accumulation of ROS that are not efficiently scavenged by increased levels of antioxidant enzymes. High levels of ROS alter the redox status and are detrimental to seed viability. Reducing ROS to appropriate concentrations is an efficient way to reduce desiccation damage and improve germination rates of recalcitrant seeds.
学科主题	Plant Sciences
WOS关键词	NITRIC-OXIDE ; SUPEROXIDE-DISMUTASE ; HYDROGEN-PEROXIDE ; PROTEIN OXIDATION ; ABIOTIC STRESS ; ACTIVE OXYGEN ; CELL-DEATH ; PLANTS ; ARABIDOPSIS ; ANTIOXIDANTS
WOS研究方向	Plant Sciences
语种	英语
WOS记录号	WOS:000287666300012
资助机构	CAS; National Science Foundation of China[30871704, 30971452, 30900871]
公开日期	2012-03-16
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn:8080/handle/151853/3834]
专题	昆明植物研究所_中国科学院东亚植物多样性与生物地理学重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Biodivers & Biogeog, Kunming Inst Bot, Inst Tibet Plateau Res Kunming, Kunming 650204, Peoples R China 2.Anhui Agr Univ, Key Lab Tea Biochem & Biotechnol, Hefei 230036, Anhui, Peoples R China 3.Huaiyin Normal Univ, Sch Life Sci, Huaiyin 223300, Peoples R China 4.Univ Kashmir, AS Coll, Dept Bot, Srinagar 190008, Jammu & Kashmir, India
推荐引用方式 GB/T 7714	Chen, Qi,Yang, Liming,Ahmad, Parvaiz,et al. Proteomic profiling and redox status alteration of recalcitrant tea (Camellia sinensis) seed in response to desiccation[J]. PLANTA,2011,233(3):583-592.
APA	Chen, Qi,Yang, Liming,Ahmad, Parvaiz,Wan, Xiaochun,&Hu, Xiangyang.(2011).Proteomic profiling and redox status alteration of recalcitrant tea (Camellia sinensis) seed in response to desiccation.PLANTA,233(3),583-592.
MLA	Chen, Qi,et al."Proteomic profiling and redox status alteration of recalcitrant tea (Camellia sinensis) seed in response to desiccation".PLANTA 233.3(2011):583-592.