CAN WE USE PBS AS AN ALTERNATIVE TO DEXTRAN IN EKTACYTOMETRIC MEASUREMENT

	CAN WE USE PBS AS AN ALTERNATIVE TO DEXTRAN IN EKTACYTOMETRIC MEASUREMENT
	WEN, ZY ; TAO, ZL ; CAO, S ; ZHAO, YH ; HAN, ZS ; LI, YM ; LU, SH ; WANG, HR ; XU, JL ; LI, G
刊名	CLINICAL HEMORHEOLOGY
	1991
卷号	11 期号:5 页码:459-464
关键词	DEFORMABILITY SUSPENDING MEDIUM EKTACYTOMETRIC
ISSN号	0271-5198
英文摘要	In order to find a new suspending medium for Ektacytometric measurement, comparisons of measured rheological properties of erythrocyte suspension in different buffers were carried out. Two kinds of buffer were used, e. g. PBS and PVP. And four types of erythrocyte were measured by using both suspeneding media, namely: normal rabbit's erythrocytes (RBC). rabbit's RBC hardened by formalin with different concentrations, rabbit's RBC treated by trypsin and human erythrocyte sampled from patients. Microscopic and scanning electron microscopic photographs of erythrocytes rigidizied al constant shear rate were observed. Results showed that (1) for every one of the these four kinds of erythrocytes, measured deformation index (D1) - shear rate (gamma) curves in PBS are similar qualitatively to those measured in PVP. (2) Microscopic and scanning electron microscopic photographs showed that erythrocytes indeed deformed when the shear rate of flow field for PBS was as low as gamma = 20s-1. It indicated that PBS could be used as suspending medium. On the contrary when we used PVP (eta = 2 mPas Fig. 1 [1]) as suspending medium at gamma = 800s-1, no obvious deformation could be obtained by using Ektacytometer. It seems to mean that, as the suspending medium for Ektacytometric measurement, PBS would be better than PVP, especially in low shear stress area. This point of view is different from that of previous works [2] which do not consider PBS as suspending medium of Ektacytometer.
WOS研究方向	Hematology
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:A1991GM64600022
内容类型	期刊论文
源URL	[http://ir.amss.ac.cn/handle/2S8OKBNM/28156]
专题	中国科学院数学与系统科学研究院
通讯作者	WEN, ZY
作者单位	1.BEIJING MED UNIV,DEPT BIOPHYS,BEIJING,PEOPLES R CHINA 2.CHINESE ACAD SCI,INST MATH,BEIJING,PEOPLES R CHINA
推荐引用方式 GB/T 7714	WEN, ZY,TAO, ZL,CAO, S,et al. CAN WE USE PBS AS AN ALTERNATIVE TO DEXTRAN IN EKTACYTOMETRIC MEASUREMENT[J]. CLINICAL HEMORHEOLOGY,1991,11(5):459-464.
APA	WEN, ZY.,TAO, ZL.,CAO, S.,ZHAO, YH.,HAN, ZS.,...&WU, BJ.(1991).CAN WE USE PBS AS AN ALTERNATIVE TO DEXTRAN IN EKTACYTOMETRIC MEASUREMENT.CLINICAL HEMORHEOLOGY,11(5),459-464.
MLA	WEN, ZY,et al."CAN WE USE PBS AS AN ALTERNATIVE TO DEXTRAN IN EKTACYTOMETRIC MEASUREMENT".CLINICAL HEMORHEOLOGY 11.5(1991):459-464.