AMPK Inhibition Enhances the Neurotoxicity of Cu(II) in SH-SY5Y Cells

doi:10.1007/s12640-016-9651-3

	AMPK Inhibition Enhances the Neurotoxicity of Cu(II) in SH-SY5Y Cells
	Hu Y(胡毅); Lan AP(兰爱平); Xiong XJ(熊先佳); Chen J(陈俊); Hu, Y; Chai, ZF; Wang, X; Chen, J; Xiong, XJ; Lan, AP
刊名	NEUROTOXICITY RESEARCH
	2016
卷号	30 期号:3 页码:499
关键词	Copper AMPK Apoptosis Neurotoxicity SH-SY5Y
DOI	10.1007/s12640-016-9651-3
通讯作者	胡毅
英文摘要	The involvement of copper in the pathophysiology of neurodegenerative disorders has been documented but remains poorly understood. This study aimed at investigating the molecular mechanism underlying copper-induced neurotoxicity. Human neuroblastoma SH-SY5Y cells were treated with different concentrations of Cu(II) (25-800 mu M). The relative levels of AMPK alpha, phosphorylated (p)-AMPK alpha were examined by western blotting. The results showed that copper reduced cell viability and enhanced apoptosis of SH-SY5Y cells. Pretreatment with N-acetyl-l-cysteine, a common ROS scavenger, decreased copper-induced cytotoxicity. Furthermore, the levels of p-AMPK alpha in SH-SY5Y cells were increased by a relatively low concentration of copper and decreased by a relatively high concentration of copper at 24 h. Moreover, inhibition of AMPK with compound C or RNA interference aggravated concentration-dependent cytotoxicity of Cu(II). Taken together, these results indicated that AMPK activity might be important for the neurotoxicity of Cu(II).
语种	英语
WOS记录号	WOS:000383727300015
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/261077]
专题	高能物理研究所_院士
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Hu Y,Lan AP,Xiong XJ,et al. AMPK Inhibition Enhances the Neurotoxicity of Cu(II) in SH-SY5Y Cells[J]. NEUROTOXICITY RESEARCH,2016,30(3):499.
APA	胡毅.,兰爱平.,熊先佳.,陈俊.,Hu, Y.,...&柴之芳.(2016).AMPK Inhibition Enhances the Neurotoxicity of Cu(II) in SH-SY5Y Cells.NEUROTOXICITY RESEARCH,30(3),499.
MLA	胡毅,et al."AMPK Inhibition Enhances the Neurotoxicity of Cu(II) in SH-SY5Y Cells".NEUROTOXICITY RESEARCH 30.3(2016):499.