Structural basis for the interaction of BamB with the POTRA3-4 domains of BamA

doi:10.1107/S2059798315024729

	Structural basis for the interaction of BamB with the POTRA3-4 domains of BamA
	Chen Z(陈真); Zhan LH(詹利红); Hou HF(侯海峰); Gao ZQ(高增强); Xu JH(徐建华); Dong C(董城); Dong YH(董宇辉); Chen, Z; Zhan, LH; Hou, HF
刊名	ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
	2016
卷号	72 期号:2 页码:236-244
关键词	outer membrane proteins BamB POTRA domains assembly and insertion crystal structure
DOI	10.1107/S2059798315024729
通讯作者	董城 ; 董宇辉
文献子类	期刊论文
英文摘要	In Escherichia coli, the Omp85 protein BamA and four lipoproteins (BamBCDE) constitute the BAMcomplex, which is essential for the assembly and insertion of outer membrane proteins into the outer membrane. Here, the crystal structure of BamB in complex with the POTRA3-4 domains of BamA is reported at 2.1 angstrom resolution. Based on this structure, the POTRA3 domain is associated with BamB via hydrogen-bonding and hydrophobic interactions. Structural and biochemical analysis revealed that the conserved residues Arg77, Glu127, Glu150, Ser167, Leu192, Leu194 and Arg195 of BamB play an essential role in interaction with the POTRA3 domain.
WOS关键词	BARREL ASSEMBLY MACHINERY ; OUTER-MEMBRANE PROTEINS ; ESCHERICHIA-COLI BAMB ; CRYSTAL-STRUCTURE ; ESSENTIAL COMPONENT ; PERIPLASMIC DOMAIN ; YAET COMPLEX ; BIOGENESIS ; ROLES ; FLEXIBILITY
语种	英语
WOS记录号	WOS:000373718400006
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/260430]
专题	高能物理研究所_多学科研究中心
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Chen Z,Zhan LH,Hou HF,et al. Structural basis for the interaction of BamB with the POTRA3-4 domains of BamA[J]. ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY,2016,72(2):236-244.
APA	陈真.,詹利红.,侯海峰.,高增强.,徐建华.,...&Dong, YH.(2016).Structural basis for the interaction of BamB with the POTRA3-4 domains of BamA.ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY,72(2),236-244.
MLA	陈真,et al."Structural basis for the interaction of BamB with the POTRA3-4 domains of BamA".ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY 72.2(2016):236-244.