CryoEM structure of yeast cytoplasmic exosome complex

doi:10.1038/cr.2016.56

	CryoEM structure of yeast cytoplasmic exosome complex
	Liu, JJ; Niu, CY; Wu, Y; Tan, D; Wang, Y; Ye, MD; Liu, Y; Zhao, WW; Zhou, K; Liu, QS
刊名	CELL RESEARCH
	2016
卷号	26 期号:7 页码:822-837
关键词	exosome RNA decay Ski7 Rrp6 cryo-EM
DOI	10.1038/cr.2016.56
文献子类	期刊论文
英文摘要	The eukaryotic multi-subunit RNA exosome complex plays crucial roles in 3'-to-5' RNA processing and decay. Rrp6 and Ski7 are the major cofactors for the nuclear and cytoplasmic exosomes, respectively. In the cytoplasm, Ski7 helps the exosome to target mRNAs for degradation and turnover via a through-core pathway. However, the interaction between Ski7 and the exosome complex has remained unclear. The transaction of RNA substrates within the exosome is also elusive. In this work, we used single-particle cryo-electron microscopy to solve the structures of the Ski7-exosome complex in RNA-free and RNA-bound forms at resolutions of 4.2 angstrom and 5.8 angstrom, respectively. These structures reveal that the N-terminal domain of Ski7 adopts a structural arrangement and interacts with the exosome in a similar fashion to the C-terminal domain of nuclear Rrp6. Further structural analysis of exosomes with RNA substrates harboring 3' overhangs of different length suggests a switch mechanism of RNA-induced exosome activation in the through-core pathway of RNA processing.
WOS关键词	MESSENGER-RNA DECAY ; MOLECULAR-DYNAMICS ; ELECTRON-MICROSCOPY ; CRYSTAL-STRUCTURE ; QUALITY-CONTROL ; NUCLEAR EXOSOME ; SKI COMPLEX ; DEGRADATION ; EXORIBONUCLEASE ; RECRUITMENT
语种	英语
WOS记录号	WOS:000379495300010
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/260389]
专题	高能物理研究所_多学科研究中心
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Liu, JJ,Niu, CY,Wu, Y,et al. CryoEM structure of yeast cytoplasmic exosome complex[J]. CELL RESEARCH,2016,26(7):822-837.
APA	Liu, JJ.,Niu, CY.,Wu, Y.,Tan, D.,Wang, Y.,...&刘全胜.(2016).CryoEM structure of yeast cytoplasmic exosome complex.CELL RESEARCH,26(7),822-837.
MLA	Liu, JJ,et al."CryoEM structure of yeast cytoplasmic exosome complex".CELL RESEARCH 26.7(2016):822-837.