A kinetic model of transcription initiation by RNA polymerase | |
Xue, Xiao-chuan; Liu, Fei; Ou-Yang, Zhong-can; Xue, XC , Tsinghua Univ, Ctr Adv Study, Beijing 100084, Peoples R China | |
刊名 | JOURNAL OF MOLECULAR BIOLOGY |
2008 | |
卷号 | 378期号:3页码:520-529 |
关键词 | Single-molecule Analysis Escherichia-coli Abortive Initiation Promoter Clearance In-vitro Elongation Complex Bubble Collapse Dna Escape Mechanism |
ISSN号 | 0022-2836 |
英文摘要 | We establish a sequence-dependent kinetic model for the later stage of transcription initiation by RNA polymerase. We suggest that there are three reaction pathways, the abortive pathway, the scrunching pathway and the escape pathway, competitive with each other at, each site during the transcription initiation. Using this three-pathway model, we mainly calculate the maximum sizes of the abortive transcripts, the abortive probabilities and the abortive/productive ratios for different promoters by Monte Carlo simulation and analytical methods. These results are quantitatively comparable with the experimental observations. In particular, our model can account for the unproductive initial transcribing complex and the nucleoside triphosphate concentration dependence of the transcription initiation, which have been found in the experiments and were hardly understood by the previous two-pathway kinetic competition model. (C) 2008 Elsevier Ltd. All rights reserved. |
学科主题 | Physics |
URL标识 | 查看原文 |
WOS记录号 | WOS:000255965600004 |
公开日期 | 2012-08-02 |
内容类型 | 期刊论文 |
源URL | [http://ir.itp.ac.cn/handle/311006/5535] |
专题 | 理论物理研究所_理论物理所1978-2010年知识产出 |
通讯作者 | Xue, XC , Tsinghua Univ, Ctr Adv Study, Beijing 100084, Peoples R China |
推荐引用方式 GB/T 7714 | Xue, Xiao-chuan,Liu, Fei,Ou-Yang, Zhong-can,et al. A kinetic model of transcription initiation by RNA polymerase[J]. JOURNAL OF MOLECULAR BIOLOGY,2008,378(3):520-529. |
APA | Xue, Xiao-chuan,Liu, Fei,Ou-Yang, Zhong-can,&Xue, XC , Tsinghua Univ, Ctr Adv Study, Beijing 100084, Peoples R China.(2008).A kinetic model of transcription initiation by RNA polymerase.JOURNAL OF MOLECULAR BIOLOGY,378(3),520-529. |
MLA | Xue, Xiao-chuan,et al."A kinetic model of transcription initiation by RNA polymerase".JOURNAL OF MOLECULAR BIOLOGY 378.3(2008):520-529. |
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