Detection of carbohydrates using new labeling reagent 1-(2-naphthyl)-3-methyl-5-pyrazolone by capillary zone electrophoresis with absorbance (UV)

	Detection of carbohydrates using new labeling reagent 1-(2-naphthyl)-3-methyl-5-pyrazolone by capillary zone electrophoresis with absorbance (UV)
	You, Jinmao 1,2; Sheng, Xiao 2; Ding, Chenxu 1; Sun, Zhiwei 2; Suo, Yourui 1; Wang, Honglun 1; Li, Win 1
刊名	analytica chimica acta
	2008-02-18
卷号	609 期号:1 页码:66-75
关键词	capillary electrophoresis (CE) carbohydrates 1-(2-naphthyl)-3-methyl-5-pyrazolone (NMP) derivatization
合作状况	其它
中文摘要	a novel labeling reagent 1-(2-naphthyl)-3-methyl-5-pyrazolone (nmp) coupled with capillary electrophoresis (ce) with dad detection for the determination of carbohydrates has been developed. the chromophore in the 1-phenyl-3-methyl-5-pyrazolone (pmp) reagent is replaced by naphthyl functional group, which results in a reagent with very high molar absorptivity (epsilon(251nm) = 5.58 x 10(4) l mol(-1) cm(-1)). this pen-nits nmp-labeled carbohydrates to be detected with uv absorbance in standard 50-mu m-i.d. fused silica capillaries by zone electrophoresis. in this mode, nanomolar concentrations of detection limits are obtained. the method for the derivatization. of carbohydrates with nmp is simplified. the derivatization reaction is rapid and mild in the presence of ammonia catalyst without further transfer steps. nine monosaccharide derivatives such as mannose, galacturonic acid, glucuronic acid, rhamnose, glucose, galactose, xylose, arabinose and fucose can successfully be detected in ce mode. good reproducibility can be obtained with relative standard deviation (r.s.d.) values of the migration times and peak area, respectively, from 0.44 to 0.48 and from 3.2 to 4.8. furthermore, the developed method has been successfully applied to the analysis of carbohydrates in the hydrolyzed rape bee pollen samples. (c) 2008 published by elsevier b.v.
英文摘要	a novel labeling reagent 1-(2-naphthyl)-3-methyl-5-pyrazolone (nmp) coupled with capillary electrophoresis (ce) with dad detection for the determination of carbohydrates has been developed. the chromophore in the 1-phenyl-3-methyl-5-pyrazolone (pmp) reagent is replaced by naphthyl functional group, which results in a reagent with very high molar absorptivity (epsilon(251nm) = 5.58 x 10(4) l mol(-1) cm(-1)). this pen-nits nmp-labeled carbohydrates to be detected with uv absorbance in standard 50-mu m-i.d. fused silica capillaries by zone electrophoresis. in this mode, nanomolar concentrations of detection limits are obtained. the method for the derivatization. of carbohydrates with nmp is simplified. the derivatization reaction is rapid and mild in the presence of ammonia catalyst without further transfer steps. nine monosaccharide derivatives such as mannose, galacturonic acid, glucuronic acid, rhamnose, glucose, galactose, xylose, arabinose and fucose can successfully be detected in ce mode. good reproducibility can be obtained with relative standard deviation (r.s.d.) values of the migration times and peak area, respectively, from 0.44 to 0.48 and from 3.2 to 4.8. furthermore, the developed method has been successfully applied to the analysis of carbohydrates in the hydrolyzed rape bee pollen samples. (c) 2008 published by elsevier b.v.
学科主题	生物科学
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	performance liquid-chromatography ; desorption/ionization mass-spectrometry ; fast-atom-bombardment ; 1-phenyl-3-methyl-5-pyrazolone derivatives ; structural characterization ; sugar chains ; oligosaccharides ; acid ; separation ; glycoproteins
收录类别	SCI
语种	英语
WOS记录号	WOS:000253560800008
公开日期	2009-12-07
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/1234]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 2.Qufu Normal Univ, Coll Chem Sci, Key Lab Life Organ Anal, Qufu 273165, Peoples R China
推荐引用方式 GB/T 7714	You, Jinmao,Sheng, Xiao,Ding, Chenxu,et al. Detection of carbohydrates using new labeling reagent 1-(2-naphthyl)-3-methyl-5-pyrazolone by capillary zone electrophoresis with absorbance (UV)[J]. analytica chimica acta,2008,609(1):66-75.
APA	You, Jinmao.,Sheng, Xiao.,Ding, Chenxu.,Sun, Zhiwei.,Suo, Yourui.,...&Li, Win.(2008).Detection of carbohydrates using new labeling reagent 1-(2-naphthyl)-3-methyl-5-pyrazolone by capillary zone electrophoresis with absorbance (UV).analytica chimica acta,609(1),66-75.
MLA	You, Jinmao,et al."Detection of carbohydrates using new labeling reagent 1-(2-naphthyl)-3-methyl-5-pyrazolone by capillary zone electrophoresis with absorbance (UV)".analytica chimica acta 609.1(2008):66-75.