Dynamic Model for Characterizing Contractile Behaviors and Mechanical Properties of a Cardiomyocyte

CORC > 沈阳自动化研究所 > 中国科学院沈阳自动化研究所 > 机器人学研究室

	Dynamic Model for Characterizing Contractile Behaviors and Mechanical Properties of a Cardiomyocyte
	He, Wenhui ; Xi N(席宁); Liu LQ(刘连庆); Wang YC(王越超); Zhang C(张闯); Wang WX(王文学)
刊名	BIOPHYSICAL JOURNAL
	2018
卷号	114 期号:1 页码:188-200
ISSN号	0006-3495
通讯作者	Wang WX(王文学) ; Liu LQ(刘连庆)
产权排序	1
中文摘要	Studies on the contractile dynamics of heart cells have attracted broad attention for the development of both heart disease therapies and cardiomyocyte-actuated micro-robotics. In this study, a linear dynamic model of a single cardiomyocyte cell was proposed at the subcellular scale to characterize the contractile behaviors of heart cells, with system parameters representing the mechanical properties of the subcellular components of living cardiomyocytes. The system parameters of the dynamic model were identified with the cellular beating pattern measured by a scanning ion conductance microscope. The experiments were implemented with cardiomyocytes in one control group and two experimental groups with the drugs cytochalasin-D or nocodazole, to identify the system parameters of the model based on scanning ion conductance microscope measurements, measurement of the cellular Young's modulus with atomic force microscopy indentation, measurement of cellular contraction forces using the micro-pillar technique, and immunofluorescence staining and imaging of the cytoskeleton. The proposed mathematical model was both indirectly and qualitatively verified by the variation in cytoskeleton, beating amplitude, and contractility of cardiomyocytes among the control and the experimental groups, as well as directly and quantitatively validated by the simulation and the significant consistency of 90.5% in the comparison between the ratios of the Young's modulus and the equivalent comprehensive cellular elasticities of cells in the experimental groups to those in the control group. Apart from mechanical properties (mass, elasticity, and viscosity) of subcellular structures, other properties of cardiomyocytes have also been studied, such as the properties of the relative action potential pattern and cellular beating frequency. This work has potential implications for research on cytobiology, drug screening, mechanisms of the heart, and cardiomyocyte-based bio-syncretic robotics.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biophysics
研究领域[WOS]	Biophysics
关键词[WOS]	ATOMIC-FORCE MICROSCOPY ; CELL-DERIVED CARDIOMYOCYTES ; CARDIAC CONSTRUCTS ; HEART-DISEASE ; MUSCLE ; MECHANOTRANSDUCTION ; SUBSTRATE ; STIFFNESS ; BIOACTUATORS ; CYTOSKELETON
收录类别	SCI
语种	英语
WOS记录号	WOS:000419595500020
内容类型	期刊论文
源URL	[http://ir.sia.cn/handle/173321/21499]
专题	沈阳自动化研究所_机器人学研究室
作者单位	1.Emerging Technologies Institute, Department of Industrial and Manufacturing Systems Engineering, University of Hong Kong Pokfulam, Pokfulam, Hong Kong 2.Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang, China 3.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China 4.University of Chinese Academy of Sciences, Beijing, China
推荐引用方式 GB/T 7714	He, Wenhui,Xi N,Liu LQ,et al. Dynamic Model for Characterizing Contractile Behaviors and Mechanical Properties of a Cardiomyocyte[J]. BIOPHYSICAL JOURNAL,2018,114(1):188-200.
APA	He, Wenhui,Xi N,Liu LQ,Wang YC,Zhang C,&Wang WX.(2018).Dynamic Model for Characterizing Contractile Behaviors and Mechanical Properties of a Cardiomyocyte.BIOPHYSICAL JOURNAL,114(1),188-200.
MLA	He, Wenhui,et al."Dynamic Model for Characterizing Contractile Behaviors and Mechanical Properties of a Cardiomyocyte".BIOPHYSICAL JOURNAL 114.1(2018):188-200.