Ultrasound Imaging Based on Molecular Targeting for Quantitative Evaluation of Hepatic Ischemia-Reperfusion Injury

doi:10.1111/ajt.14345

CORC > 自动化研究所 > 中国科学院自动化研究所 > 中国科学院分子影像重点实验室

	Ultrasound Imaging Based on Molecular Targeting for Quantitative Evaluation of Hepatic Ischemia-Reperfusion Injury
	Chen Qiu ; Tinghui Yina ; Yingcai Zhang ; Yufan Lian ; Yujia You ; Kun Wang; Rongqin Zheng ; Xintao Shuai
刊名	American Journal of Transplantation
	2017
卷号	17 期号:12 页码:3087–3097
关键词	Ultrasound Imaging Hepatic Ischemia Reperfusion Injury Molecular Targeting
DOI	10.1111/ajt.14345
英文摘要	The present study aimed to quantitatively diagnose and monitor the therapy response of hepatic ischemia reperfusion injury (IRI) with targeted ultrasound (US) imaging. Targeted microbubbles (MBs) were fabricated, and the binding of ICAM-1 antibodies to MBs was observed. To establish a quantitative method based on targeted US imaging, contrast-enhanced ultrasound (CEUS) was applied on IRI rats. After andrographolide treatment, the IRI rats were subjected to the quantitative targeted US imaging for a therapeutic effect. Effective binding of ICAM-1 antibodies to MBs was observed. According to the quantitative targeted US imaging, the ICAM-1 normalized intensity difference (NID) in IRI rats (38.74 ± 15.08%) was significantly higher than that in the control rats (10.08 ± 2.52%, P = 0.048). Furthermore, different degrees of IRI (mild IRI, moderate to severe IRI) were distinguished by the use of NID (37.14 ± 2.14, 22.34 ± 1.08, P = 0.002). Analysis of mRNA expression demonstrated the accuracy of analyzing the NID using quantitative targeted US imaging (R2 = 0.7434, P < 0.001). Andrographolide treatment resulted in obviously weakened NID of ICAM-1 (17.7 ± 4.8% vs 34.2 ± 6.6%, P < 0.001). The study showed the potential of the quantitative targeted US imaging method for the diagnosis and therapeutic monitoring of IRI.
WOS记录号	WOS:000418567000014
内容类型	期刊论文
源URL	[http://ir.ia.ac.cn/handle/173211/19562]
专题	自动化研究所_中国科学院分子影像重点实验室
作者单位	1.Department of Medical Ultrasound, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China. 2.Guangdong Key Laboratory of Liver Disease Research, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China. 3.Department of Liver Transplantation, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China 4.Key Laboratory of Molecular Imaging, Institute of Automation, Chinese Academy of Science, Beijing 100190, China 5.PCFM Lab of Ministry of Education, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China. 6.Center for Biomedical Engineering, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510275, China.
推荐引用方式 GB/T 7714	Chen Qiu,Tinghui Yina,Yingcai Zhang,et al. Ultrasound Imaging Based on Molecular Targeting for Quantitative Evaluation of Hepatic Ischemia-Reperfusion Injury[J]. American Journal of Transplantation,2017,17(12):3087–3097.
APA	Chen Qiu.,Tinghui Yina.,Yingcai Zhang.,Yufan Lian.,Yujia You.,...&Xintao Shuai.(2017).Ultrasound Imaging Based on Molecular Targeting for Quantitative Evaluation of Hepatic Ischemia-Reperfusion Injury.American Journal of Transplantation,17(12),3087–3097.
MLA	Chen Qiu,et al."Ultrasound Imaging Based on Molecular Targeting for Quantitative Evaluation of Hepatic Ischemia-Reperfusion Injury".American Journal of Transplantation 17.12(2017):3087–3097.