Stripe artifact elimination based on nonsubsampled contourlet transform for light sheet fluorescence microscopy
Liang, Xiao1,2,3; Zang, Yali1,2,3; Dong, Di1,2,3; Zhang, Liwen1,2; Fang, Mengjie1,2,3; Yang, Xin1,2,3; Arranz, Alicia4; Ripoll, Jorge5,6; Hui, Hui1,2,3; Tian, Jie1,2,3
刊名JOURNAL OF BIOMEDICAL OPTICS
2016-10-01
卷号21期号:10页码:1-9
关键词Light Sheet Fluorescence Microscopy Nonsubsampled Contourlet Transform Stripe Artifact
DOI10.1117/1.JBO.21.10.106005
文献子类Article
英文摘要Stripe artifacts, caused by high-absorption or high-scattering structures in the illumination light path, are a common drawback in both unidirectional and multidirectional light sheet fluorescence microscopy (LSFM), significantly deteriorating image quality. To circumvent this problem, we present an effective multidirectional stripe remover (MDSR) method based on nonsubsampled contourlet transform (NSCT), which can be used for both unidirectional and multidirectional LSFM. In MDSR, a fast Fourier transform (FFT) filter is designed in the NSCT domain to shrink the stripe components and eliminate the noise. Benefiting from the properties of being multiscale and multidirectional, MDSR succeeds in eliminating stripe artifacts in both unidirectional and multidirectional LSFM. To validate the method, MDSR has been tested on images from a custom-made unidirectional LSFM system and a commercial multidirectional LSFM system, clearly demonstrating that MDSR effectively removes most of the stripe artifacts. Moreover, we performed a comparative experiment with the variational stationary noise remover and the wavelet-FFT methods and quantitatively analyzed the results with a peak signal-to-noise ratio, showing an improved noise removal when using the MDSR method. (C) 2016 Society of Photo-Optical Instrumentation Engineers (SPIE)
WOS关键词PLANE ILLUMINATION MICROSCOPY ; SINGLE-CELL RESOLUTION ; TOMOGRAPHY ; ALGORITHM ; IMAGES
WOS研究方向Biochemistry & Molecular Biology ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
语种英语
WOS记录号WOS:000388213300028
资助机构National Natural Science Foundation of China under Grant Nos. 81227901, 61231004, 81501616, 81301346, 81527805, and 81671851 ; Instrument Developing Project (Nos. YZ201502 and YZ201457) ; Strategic Priority Research Program of the CAS(XDB02060010) ; Beijing Municipal Science and Technology Commission(Z161100002616022) ; Marie Curie Intra-European Fellowship Program(IEF-2010-275137) ; EC FP7 CIG grant HIGH-THROUGHPUT TOMO ; Spanish MINECO project(FIS2013-41802-R MESO-IMAGING)
内容类型期刊论文
源URL[http://ir.ia.ac.cn/handle/173211/13355]  
专题自动化研究所_中国科学院分子影像重点实验室
作者单位1.Chinese Acad Sci, Key Lab Mol Imaging, 95 Zhongguancun East Rd, Beijing 100190, Peoples R China
2.State Key Lab Management & Control Complex Syst, 95 Zhongguancun East Rd, Beijing 100190, Peoples R China
3.Univ Chinese Acad Sci, 80 Zhongguancun East Rd, Beijing 100190, Peoples R China
4.Ctr Mol Biol Severo Ochoa, Calle Nicolas Cabrera 1, Madrid 28049, Spain
5.Univ Carlos III Madrid, Dept Bioengn & Aerosp Engn, Escuela Politecn Super, Ave Univ 30, Madrid 28911, Spain
6.Hosp Gregorio Maranon, Inst Invest Sanitaria, Expt Med & Surg Unit, Calle Dr Esquerdo 46, Madrid 28007, Spain
推荐引用方式
GB/T 7714
Liang, Xiao,Zang, Yali,Dong, Di,et al. Stripe artifact elimination based on nonsubsampled contourlet transform for light sheet fluorescence microscopy[J]. JOURNAL OF BIOMEDICAL OPTICS,2016,21(10):1-9.
APA Liang, Xiao.,Zang, Yali.,Dong, Di.,Zhang, Liwen.,Fang, Mengjie.,...&Tian, Jie.(2016).Stripe artifact elimination based on nonsubsampled contourlet transform for light sheet fluorescence microscopy.JOURNAL OF BIOMEDICAL OPTICS,21(10),1-9.
MLA Liang, Xiao,et al."Stripe artifact elimination based on nonsubsampled contourlet transform for light sheet fluorescence microscopy".JOURNAL OF BIOMEDICAL OPTICS 21.10(2016):1-9.
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