Combination of focused ion beam (FIB) and microtome by ultrathin slice preparation for transmission electron microscopy (TEM) observation

doi:10.1186/s40623-018-0920-7

CORC > 地质与地球物理研究所 > 中国科学院地质与地球物理研究所 > 中国科学院地球与行星物理重点实验室

	Combination of focused ion beam (FIB) and microtome by ultrathin slice preparation for transmission electron microscopy (TEM) observation
	Xu, Yuchen 1; Gu, Lixin 1; Li, Yang 2; Mo, Bing 2; Lin, Yangting 1,3
刊名	EARTH PLANETS AND SPACE
	2018-09-19
卷号	70 页码:6
关键词	Focused ion beam Transmission electron microscopy Microtome Presolar grains Graphite
ISSN号	1880-5981
DOI	10.1186/s40623-018-0920-7
英文摘要	There are growing demands for integrated study of isotopes, trace elements and crystallography of micron-sized grains observed on polished sections or scattered on surface of sample holders. However, transmission electron microscopy (TEM) observation is largely restricted by the challenges associated with the preparation of ultrathin sections from given micron-sized grains after chemical and isotopic analyses. Here, we report a new method of combining focused ion beam (FIB) and microtome techniques. This method is demonstrated by the successful preparation of several ultrathin slices from a presolar graphite grain. The presolar graphite grain was a spherule with a diameter of 5 mu m. It was found by nanoscale secondary ion mass spectrometry (NanoSIMS) mapping of a carbonaceous acid residue from the Qingzhen enstatite chondrite (EH3), deposited on a gold foil mount. The spherule showed isotope anomalies in C and Si, with C-12/C-13= 99 +/- 2 and delta Si-29/Si-28=172 +/- 36%o, and likely originated in an asymptotic branch giant (AGB) star. After NanoSIMS analysis, this spherule was transported and fixed on a pre-cut top surface of an epoxy stub, using FIB. The fixed spherule was then embedded entirely in resin, and cut into similar to 70 nm slices with a microtome. TEM observation of these ultrathin sections revealed a turbostratic structure free of any subgrains. The morphology, Raman spectrum, C and Si isotopic compositions, internal texture and crystallography of this graphite spherule suggest that it condensed from a carbon-rich outflow of an AGB star.
资助项目	National Natural Science Foundation of China[41673069] ; National Natural Science Foundation of China[41503066] ; Key Research Program of Frontier Sciences, CAS[QYZDJ-SSW-DQC001]
WOS关键词	SITU LIFT-OUT ; SPECIMEN PREPARATION ; PRESOLAR GRAPHITE ; GRAINS ; METEORITE ; STARS
WOS研究方向	Geology
语种	英语
出版者	SPRINGEROPEN
WOS记录号	WOS:000445111600003
资助机构	National Natural Science Foundation of China ; National Natural Science Foundation of China ; Key Research Program of Frontier Sciences, CAS ; Key Research Program of Frontier Sciences, CAS ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Key Research Program of Frontier Sciences, CAS ; Key Research Program of Frontier Sciences, CAS ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Key Research Program of Frontier Sciences, CAS ; Key Research Program of Frontier Sciences, CAS ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Key Research Program of Frontier Sciences, CAS ; Key Research Program of Frontier Sciences, CAS
内容类型	期刊论文
源URL	[http://ir.iggcas.ac.cn/handle/132A11/88720]
专题	地质与地球物理研究所_中国科学院地球与行星物理重点实验室
通讯作者	Lin, Yangting
作者单位	1.Chinese Acad Sci, Inst Geol & Geophys, Key Lab Earth & Planetary Phys, 19 Beituchengxilu, Beijing 100029, Peoples R China 2.Chinese Acad Sci, Ctr Lunar & Planetary Sci, Inst Geochem, Guiyang 550081, Guizhou, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Xu, Yuchen,Gu, Lixin,Li, Yang,et al. Combination of focused ion beam (FIB) and microtome by ultrathin slice preparation for transmission electron microscopy (TEM) observation[J]. EARTH PLANETS AND SPACE,2018,70:6.
APA	Xu, Yuchen,Gu, Lixin,Li, Yang,Mo, Bing,&Lin, Yangting.(2018).Combination of focused ion beam (FIB) and microtome by ultrathin slice preparation for transmission electron microscopy (TEM) observation.EARTH PLANETS AND SPACE,70,6.
MLA	Xu, Yuchen,et al."Combination of focused ion beam (FIB) and microtome by ultrathin slice preparation for transmission electron microscopy (TEM) observation".EARTH PLANETS AND SPACE 70(2018):6.