Expression patterns of key iron and oxygen metabolism genes during magnetosome formation in Magnetospirillum gryphiswaldense MSR-1

doi:10.1111/1574-6968.12234

	Expression patterns of key iron and oxygen metabolism genes during magnetosome formation in Magnetospirillum gryphiswaldense MSR-1
	Wang, Qing 1,2; Liu, Jin-Xin 1,2; Zhang, Wei-Jia 1,3,4; Zhang, Tong-Wei 1,2,5; Yang, Jing 1,2; Li, Ying 1,2
刊名	FEMS MICROBIOLOGY LETTERS
	2013-10-01
卷号	347 期号:2 页码:163-172
关键词	Magnetospirillum gryphiswaldense iron metabolism oxygen metabolism magnetosome formation real-time polymerase chain reaction hierarchical clustering
ISSN号	0378-1097
DOI	10.1111/1574-6968.12234
文献子类	Article
英文摘要	To evaluate the expression patterns of genes involved in iron and oxygen metabolism during magnetosome formation, the profiles of 13 key genes in Magnetospirillum gryphiswaldense MSR-1 cells cultured under high-iron vs. low-iron conditions were examined. Cell growth rates did not differ between the two conditions. Only the high-iron cells produced magnetosomes. Transmission electron microscopy observations revealed that magnetosome formation began at 6h and crystal maturation occurred from 10 to 18h. Real-time polymerase chain reaction analysis showed that expression of these genes increased during cell growth and magnetosome synthesis, particularly for ferric reductase gene (fer6) and ferrous transport system-related genes feoAB1,feoAB2,sodB, and katG. The low-iron cells showed increased expression of feoAB1 and feoB2 from 12 to 18h but no clear expression changes for the other genes. Expression patterns of the genes were divided by hierarchical clustering into four clusters for the high-iron cells and three clusters for the low-iron cells. Each cluster included both iron and oxygen metabolism genes showing similar expression patterns. The findings indicate the coordination and co-dependence of iron and oxygen metabolism gene activity to achieve a balance during the biomineralization process. Future transcriptome analysis will help elucidate the mechanism of biomineralization in MSR-1 magnetosome formation.
WOS关键词	MAGNETOTACTIC BACTERIUM ; SUPEROXIDE-DISMUTASE ; STRAIN MSR-1 ; BIOMINERALIZATION ; ORGANIZATION ; MECHANISMS ; REDUCTION ; SPIRILLUM ; CULTURE ; GROWTH
WOS研究方向	Microbiology
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000325104200010
内容类型	期刊论文
源URL	[http://ir.iggcas.ac.cn/handle/132A11/87772]
专题	中国科学院地质与地球物理研究所
通讯作者	Li, Ying
作者单位	1.China Agr Univ, Coll Biol Sci, State Key Labs Agrobiotechnol, Beijing 100193, Peoples R China 2.France China Biomineralizat & Nanostruct Lab, Beijing, Peoples R China 3.Chinese Acad Med Sci, Inst Pathogen Biol, Beijing 100730, Peoples R China 4.Peking Union Med Coll, Beijing 100021, Peoples R China 5.Chinese Acad Sci, Inst Geol & Geophys, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wang, Qing,Liu, Jin-Xin,Zhang, Wei-Jia,et al. Expression patterns of key iron and oxygen metabolism genes during magnetosome formation in Magnetospirillum gryphiswaldense MSR-1[J]. FEMS MICROBIOLOGY LETTERS,2013,347(2):163-172.
APA	Wang, Qing,Liu, Jin-Xin,Zhang, Wei-Jia,Zhang, Tong-Wei,Yang, Jing,&Li, Ying.(2013).Expression patterns of key iron and oxygen metabolism genes during magnetosome formation in Magnetospirillum gryphiswaldense MSR-1.FEMS MICROBIOLOGY LETTERS,347(2),163-172.
MLA	Wang, Qing,et al."Expression patterns of key iron and oxygen metabolism genes during magnetosome formation in Magnetospirillum gryphiswaldense MSR-1".FEMS MICROBIOLOGY LETTERS 347.2(2013):163-172.