Analysis of Magnetosome Chains in Magnetotactic Bacteria by Magnetic Measurements and Automated Image Analysis of Electron Micrographs

doi:10.1128/AEM.02143-13

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	Analysis of Magnetosome Chains in Magnetotactic Bacteria by Magnetic Measurements and Automated Image Analysis of Electron Micrographs
	Katzmann, E.1,2; Eibauer, M.3; Lin, W.4; Pan, Y.4; Plitzko, J. M.2,5; Schueler, D.1
刊名	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
	2013-12-01
卷号	79 期号:24 页码:7755-7762
DOI	10.1128/AEM.02143-13
文献子类	Article
英文摘要	Magnetotactic bacteria (MTB) align along the Earth's magnetic field by the activity of intracellular magnetosomes, which are membrane-enveloped magnetite or greigite particles that are assembled into well-ordered chains. Formation of magnetosome chains was found to be controlled by a set of specific proteins in Magnetospirillum gryphiswaldense and other MTB. However, the contribution of abiotic factors on magnetosome chain assembly has not been fully explored. Here, we first analyzed the effect of growth conditions on magnetosome chain formation in M. gryphiswaldense by electron microscopy. Whereas higher temperatures (30 to 35 degrees C) and high oxygen concentrations caused increasingly disordered chains and smaller magnetite crystals, growth at 20 degrees C and anoxic conditions resulted in long chains with mature cuboctahedron-shaped crystals. In order to analyze the magnetosome chain in electron microscopy data sets in a more quantitative and unbiased manner, we developed a computerized image analysis algorithm. The collected data comprised the cell dimensions and particle size and number as well as the intracellular position and extension of the magnetosome chain. The chain analysis program (CHAP) was used to evaluate the effects of the genetic and growth conditions on magnetosome chain formation. This was compared and correlated to data obtained from bulk magnetic measurements of wild-type (WT) and mutant cells displaying different chain configurations. These techniques were used to differentiate mutants due to magnetosome chain defects on a bulk scale.
WOS关键词	MAGNETOSPIRILLUM-GRYPHISWALDENSE ; REMANENT MAGNETIZATION ; PROTEIN MAMK ; GROWTH ; ACTIN ; MEMBRANE ; ISLAND ; BIOMINERALIZATION
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
语种	英语
WOS记录号	WOS:000327814600024
资助机构	Deutsche Forschungsgemeinschaft(Schu1080/9-1 ; Deutsche Forschungsgemeinschaft(Schu1080/9-1 ; European Union(Bio2Man4MRI) ; European Union(Bio2Man4MRI) ; -15-1) ; -15-1) ; Deutsche Forschungsgemeinschaft(Schu1080/9-1 ; Deutsche Forschungsgemeinschaft(Schu1080/9-1 ; European Union(Bio2Man4MRI) ; European Union(Bio2Man4MRI) ; -15-1) ; -15-1)
内容类型	期刊论文
源URL	[http://ir.iggcas.ac.cn/handle/132A11/62647]
专题	地质与地球物理研究所_地球深部结构与过程研究室
作者单位	1.Univ Munich, Dept Biol 1, Planegg Martinsried, Germany 2.Max Planck Inst Biochem, Dept Mol Struct Biol, Planegg Martinsried, Germany 3.Univ Zurich, Dept Biochem, Zurich, Switzerland 4.Chinese Acad Sci, Inst Geol & Geophys, Key Lab Earths Deep Interior, Biogeomagnetism Grp,Paleomagnetism & Geochronol L, Beijing, Peoples R China 5.Univ Utrecht, Bijvoet Ctr Biomol Res, Utrecht, Netherlands
推荐引用方式 GB/T 7714	Katzmann, E.,Eibauer, M.,Lin, W.,et al. Analysis of Magnetosome Chains in Magnetotactic Bacteria by Magnetic Measurements and Automated Image Analysis of Electron Micrographs[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2013,79(24):7755-7762.
APA	Katzmann, E.,Eibauer, M.,Lin, W.,Pan, Y.,Plitzko, J. M.,&Schueler, D..(2013).Analysis of Magnetosome Chains in Magnetotactic Bacteria by Magnetic Measurements and Automated Image Analysis of Electron Micrographs.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,79(24),7755-7762.
MLA	Katzmann, E.,et al."Analysis of Magnetosome Chains in Magnetotactic Bacteria by Magnetic Measurements and Automated Image Analysis of Electron Micrographs".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 79.24(2013):7755-7762.