A key time point for cell growth and magnetosome synthesis of Magnetospirillum gryphiswaldense based on real-tirne analysis of physiological factors

doi:10.3389/fmicb.2013.00210

	A key time point for cell growth and magnetosome synthesis of Magnetospirillum gryphiswaldense based on real-tirne analysis of physiological factors
	Yang, Jing 1,2,3; Li, Shuqi 1,2,3; Huang, Xiuliang 1,2,3; Tang, Tao 1,2,3; Jiang, Weizhong 4; Zhang, Tongwei 1,2,5; Li, Ying 1,2,3
刊名	FRONTIERS IN MICROBIOLOGY
	2013-07-24
卷号	4
关键词	Magnetospirillum Gryphiswaldense Submerged Culture Physiological Features Magnetosome Synthesis Key Time Point
ISSN号	1664-302X
DOI	10.3389/fmicb.2013.00210
文献子类	Article
英文摘要	Pure culture of magnetotactic bacteria with high magnetosome yield has been achieved for only a few strains. The major obstacles involve the nutritional requirements and culture conditions of the cells. To increase cell density and magnetosome production, it is necessary to elucidate the physiological characteristics of a particular strain during cell growth and develop an appropriate artificial control strategy. Large-scale culture of Magnetospirillum gryphiswaldense strain MSR-1 was successfully performed for 48 h in a 42-L autofermentor, and several key physiological parameters were measured in real time. Maximal values of cell density (OD565) (19.4) and cell yield (dry weight) (4.76 g/L) were attained at 40 h. The key time point for cell growth and magnetosome formation was found to be 18-20 h. At this point, cells entered the log phase of growth, the maximal values of Cmag (1.78), iron content (0.47%), and magnetosome number (26 +/- 3 per cell) were observed, superoxide dismutase (SOD) activity began to decrease more rapidly, ATP content dropped to an extremely low level (0.17 fmol), and reducing power (NADH/NAD+ ratio) began to increase very rapidly. Excessive levels of dissolved oxygen (>= 20 ppb) and lactic acid in the medium caused notable cytotoxic effects after 20 h. Artificial control measures for fermentation must be based on realistic cell physiological conditions. At the key time point (18-20 h), cell density is high and magnetosomes have matured. The process of magnetosome synthesis involves a high consumption of ATP and reducing power, and the cells require replenishment of nutrients prior to the 18-20 h time point. Culture conditions that effectively minimize dissolved oxygen accumulation, lactic acid content, and reducing power at this point will enhance magnetosome yield without obvious inhibition of cell growth.
WOS关键词	OXYGEN-CONTROLLED FERMENTER ; MAGNETOTACTIC BACTERIA ; PROTEIN ; CULTURE ; MSR-1 ; EXPRESSION ; SPIRILLUM
WOS研究方向	Microbiology
语种	英语
出版者	FRONTIERS RESEARCH FOUNDATION
WOS记录号	WOS:000331366300001
资助机构	National Natural Science Foundation of China(31270093 ; National Natural Science Foundation of China(31270093 ; Undergraduate Innovation Program of China Agricultural University(2010-BKS-16) ; Undergraduate Innovation Program of China Agricultural University(2010-BKS-16) ; J1103520) ; J1103520) ; National Natural Science Foundation of China(31270093 ; National Natural Science Foundation of China(31270093 ; Undergraduate Innovation Program of China Agricultural University(2010-BKS-16) ; Undergraduate Innovation Program of China Agricultural University(2010-BKS-16) ; J1103520) ; J1103520) ; National Natural Science Foundation of China(31270093 ; National Natural Science Foundation of China(31270093 ; Undergraduate Innovation Program of China Agricultural University(2010-BKS-16) ; Undergraduate Innovation Program of China Agricultural University(2010-BKS-16) ; J1103520) ; J1103520) ; National Natural Science Foundation of China(31270093 ; National Natural Science Foundation of China(31270093 ; Undergraduate Innovation Program of China Agricultural University(2010-BKS-16) ; Undergraduate Innovation Program of China Agricultural University(2010-BKS-16) ; J1103520) ; J1103520)
内容类型	期刊论文
源URL	[http://ir.iggcas.ac.cn/handle/132A11/86266]
专题	中国科学院地质与地球物理研究所
通讯作者	Li, Ying
作者单位	1.China Agr Univ, Coll Biol Sci, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China 2.China Agr Univ, Coll Biol Sci, Minist Agr, Key Lab Soil Microbiol, Beijing 100193, Peoples R China 3.France China Biomineralizat & Nanostruct Lab, Beijing, Peoples R China 4.China Agr Univ, Coll Water Resources & Civil Engn, Beijing 100193, Peoples R China 5.Chinese Acad Sci, Inst Geol & Geophys, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Yang, Jing,Li, Shuqi,Huang, Xiuliang,et al. A key time point for cell growth and magnetosome synthesis of Magnetospirillum gryphiswaldense based on real-tirne analysis of physiological factors[J]. FRONTIERS IN MICROBIOLOGY,2013,4.
APA	Yang, Jing.,Li, Shuqi.,Huang, Xiuliang.,Tang, Tao.,Jiang, Weizhong.,...&Li, Ying.(2013).A key time point for cell growth and magnetosome synthesis of Magnetospirillum gryphiswaldense based on real-tirne analysis of physiological factors.FRONTIERS IN MICROBIOLOGY,4.
MLA	Yang, Jing,et al."A key time point for cell growth and magnetosome synthesis of Magnetospirillum gryphiswaldense based on real-tirne analysis of physiological factors".FRONTIERS IN MICROBIOLOGY 4(2013).