Magnetosomes extracted from Magnetospirillum magneticum strain AMB-1 showed enhanced peroxidase-like activity under visible-light irradiation

doi:10.1016/j.enzmictec.2015.02.009

	Magnetosomes extracted from Magnetospirillum magneticum strain AMB-1 showed enhanced peroxidase-like activity under visible-light irradiation
	Li, Kefeng 1,2,3; Chen, Chuanfang 1,4; Chen, Changyou 1,2; Wang, Yuzhan 1; Wei, Zhao 1,2; Pan, Weidong 1,4; Song, Tao 1,4
刊名	ENZYME AND MICROBIAL TECHNOLOGY
	2015-05-01
卷号	72 页码:72-78
关键词	Magnetosome Peroxidase-like activity Urea hydrogen peroxide Light irradiation
ISSN号	0141-0229
DOI	10.1016/j.enzmictec.2015.02.009
文献子类	Article
英文摘要	Magnetosomes are intracellular structures produced by magnetotactic bacteria and are magnetic nanoparticles surrounded by a lipid bilayer membrane. Magnetosomes reportedly possess intrinsic enzyme mimetic activity similar to that found in horseradish peroxidase (HRP) and can scavenge reactive oxygen species depending on peroxidase activity. Our previous study has demonstrated the phototaxis characteristics of Magnetospirillum magneticum strain AMB-1 cells, but the mechanism is not well understood. Therefore, we studied the relationship between visible-light irradiation and peroxidase-like activity of magnetosomes extracted from M. magneticum strain AMB-1. We then compared this characteristic with that of HRP, iron ions, and naked magnetosomes using 3,3',5,5'-tetramethylbenzidine as a peroxidase substrate in the presence of H2O2. Results showed that HRP and iron ions had different activities from those of magnetosomes and naked magnetosomes when exposed to visible-light irradiation. Magnetosomes and naked magnetosomes had enhanced peroxidase-like activities under visible-light irradiation, but magnetosomes showed less affinity toward substrates than naked magnetosomes under visible-light irradiation. These results suggested that the peroxidase-like activity of magnetosomes may follow an ordered ternary mechanism rather than a ping-pong mechanism. This finding may provide new insight into the function of magnetosomes in the phototaxis in magnetotactic bacteria. (C) 2015 Elsevier Inc. All rights reserved.
WOS关键词	MAGNETOTACTIC BACTERIA ; AQUASPIRILLUM-MAGNETOTACTICUM ; HORSERADISH-PEROXIDASE ; SP-NOV ; OXIDATION ; WATER ; NANOPARTICLES ; SUBSTRATE
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:000353095400010
资助机构	State Key Program of National Natural Science Foundation of China(51037006) ; State Key Program of National Natural Science Foundation of China(51037006) ; National Basic Research Program of China(2011CB503702) ; National Basic Research Program of China(2011CB503702) ; State Key Program of National Natural Science Foundation of China(51037006) ; State Key Program of National Natural Science Foundation of China(51037006) ; National Basic Research Program of China(2011CB503702) ; National Basic Research Program of China(2011CB503702) ; State Key Program of National Natural Science Foundation of China(51037006) ; State Key Program of National Natural Science Foundation of China(51037006) ; National Basic Research Program of China(2011CB503702) ; National Basic Research Program of China(2011CB503702) ; State Key Program of National Natural Science Foundation of China(51037006) ; State Key Program of National Natural Science Foundation of China(51037006) ; National Basic Research Program of China(2011CB503702) ; National Basic Research Program of China(2011CB503702)
内容类型	期刊论文
源URL	[http://ir.iggcas.ac.cn/handle/132A11/84052]
专题	中国科学院地质与地球物理研究所
通讯作者	Song, Tao
作者单位	1.Chinese Acad Sci, Inst Elect Engn, Beijing Key Lab Bioelectromagnetism, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Shandong Sport Univ, Jinan 250102, Peoples R China 4.France China Biomineralizat & Nanostruct Lab, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Li, Kefeng,Chen, Chuanfang,Chen, Changyou,et al. Magnetosomes extracted from Magnetospirillum magneticum strain AMB-1 showed enhanced peroxidase-like activity under visible-light irradiation[J]. ENZYME AND MICROBIAL TECHNOLOGY,2015,72:72-78.
APA	Li, Kefeng.,Chen, Chuanfang.,Chen, Changyou.,Wang, Yuzhan.,Wei, Zhao.,...&Song, Tao.(2015).Magnetosomes extracted from Magnetospirillum magneticum strain AMB-1 showed enhanced peroxidase-like activity under visible-light irradiation.ENZYME AND MICROBIAL TECHNOLOGY,72,72-78.
MLA	Li, Kefeng,et al."Magnetosomes extracted from Magnetospirillum magneticum strain AMB-1 showed enhanced peroxidase-like activity under visible-light irradiation".ENZYME AND MICROBIAL TECHNOLOGY 72(2015):72-78.