Recover vigorous cells of Magnetospirillum magneticum AMB-1 by capillary magnetic separation | |
Li Jinhua1,2; Ge Xin1; Zhang Xiaokui3; Chen Guanjun1; Pan Yongxin2 | |
刊名 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY |
2010-07-01 | |
卷号 | 28期号:4页码:826-831 |
关键词 | magnetotactic bacteria Magnetospirillum magneticum AMB-1 capillary magnetic separation strain recovery |
ISSN号 | 0254-4059 |
DOI | 10.1007/s00343-010-9068-4 |
文献子类 | Article |
英文摘要 | Cultivable magnetotactic bacteria (MTB) in laboratory can provide sufficient samples for molecular microbiological and magnetic studies. However, a cold-stored MTB strain, such as Magnetospirillum magneticum AMB-1, often loses its ability to synthesize magnetosomes and consequently fails to sense the external magnetic field. It is therefore important to quickly recover vigorous bacteria cells that highly capable of magnetosome producing. In this study, a modified capillary magnetic separation system was designed to recover a deteriorating strain of Magnetospirillum magneticum AMB-1 that long-term cold-stored in a refrigerator. The results show that all cells obtained after a 3-cycle treatment were vigorous and had the ability to produce magnetosomes. Moreover, the 3(rd)-cycle recovered cells were able to form more magnetosome crystals. Compared with the colony formation method, this new method is time-saving, easily operated, and more efficient for recovering vigorous MTB cells. |
WOS关键词 | MAGNETOTACTIC BACTERIA ; MAGNETOSOME ; GRYPHISWALDENSE ; ISLAND ; COCCI |
WOS研究方向 | Marine & Freshwater Biology ; Oceanography |
语种 | 英语 |
出版者 | SCIENCE PRESS |
WOS记录号 | WOS:000279294400016 |
资助机构 | Natural Science Foundation of Shandong Province, China(2006ZRB01973) ; Natural Science Foundation of Shandong Province, China(2006ZRB01973) ; National Natural Science Foundation of China(40821091 ; National Natural Science Foundation of China(40821091 ; 40325011) ; 40325011) ; Natural Science Foundation of Shandong Province, China(2006ZRB01973) ; Natural Science Foundation of Shandong Province, China(2006ZRB01973) ; National Natural Science Foundation of China(40821091 ; National Natural Science Foundation of China(40821091 ; 40325011) ; 40325011) ; Natural Science Foundation of Shandong Province, China(2006ZRB01973) ; Natural Science Foundation of Shandong Province, China(2006ZRB01973) ; National Natural Science Foundation of China(40821091 ; National Natural Science Foundation of China(40821091 ; 40325011) ; 40325011) ; Natural Science Foundation of Shandong Province, China(2006ZRB01973) ; Natural Science Foundation of Shandong Province, China(2006ZRB01973) ; National Natural Science Foundation of China(40821091 ; National Natural Science Foundation of China(40821091 ; 40325011) ; 40325011) |
内容类型 | 期刊论文 |
源URL | [http://ir.iggcas.ac.cn/handle/132A11/72486] |
专题 | 中国科学院地质与地球物理研究所 |
通讯作者 | Chen Guanjun |
作者单位 | 1.Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China 2.Chinese Acad Sci, Biogeomagnetism Grp, Paleomagnetism & Geochronol Lab SKL LE, Inst Geol & Geophys, Beijing 100029, Peoples R China 3.Shandong Univ, Marine Coll, Weihai 264209, Peoples R China |
推荐引用方式 GB/T 7714 | Li Jinhua,Ge Xin,Zhang Xiaokui,et al. Recover vigorous cells of Magnetospirillum magneticum AMB-1 by capillary magnetic separation[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2010,28(4):826-831. |
APA | Li Jinhua,Ge Xin,Zhang Xiaokui,Chen Guanjun,&Pan Yongxin.(2010).Recover vigorous cells of Magnetospirillum magneticum AMB-1 by capillary magnetic separation.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,28(4),826-831. |
MLA | Li Jinhua,et al."Recover vigorous cells of Magnetospirillum magneticum AMB-1 by capillary magnetic separation".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 28.4(2010):826-831. |
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