Recover vigorous cells of Magnetospirillum magneticum AMB-1 by capillary magnetic separation

doi:10.1007/s00343-010-9068-4

	Recover vigorous cells of Magnetospirillum magneticum AMB-1 by capillary magnetic separation
	Li Jinhua 1,2; Ge Xin 1; Zhang Xiaokui 3; Chen Guanjun 1; Pan Yongxin 2
刊名	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2010-07-01
卷号	28 期号:4 页码:826-831
关键词	magnetotactic bacteria Magnetospirillum magneticum AMB-1 capillary magnetic separation strain recovery
ISSN号	0254-4059
DOI	10.1007/s00343-010-9068-4
文献子类	Article
英文摘要	Cultivable magnetotactic bacteria (MTB) in laboratory can provide sufficient samples for molecular microbiological and magnetic studies. However, a cold-stored MTB strain, such as Magnetospirillum magneticum AMB-1, often loses its ability to synthesize magnetosomes and consequently fails to sense the external magnetic field. It is therefore important to quickly recover vigorous bacteria cells that highly capable of magnetosome producing. In this study, a modified capillary magnetic separation system was designed to recover a deteriorating strain of Magnetospirillum magneticum AMB-1 that long-term cold-stored in a refrigerator. The results show that all cells obtained after a 3-cycle treatment were vigorous and had the ability to produce magnetosomes. Moreover, the 3(rd)-cycle recovered cells were able to form more magnetosome crystals. Compared with the colony formation method, this new method is time-saving, easily operated, and more efficient for recovering vigorous MTB cells.
WOS关键词	MAGNETOTACTIC BACTERIA ; MAGNETOSOME ; GRYPHISWALDENSE ; ISLAND ; COCCI
WOS研究方向	Marine & Freshwater Biology ; Oceanography
语种	英语
出版者	SCIENCE PRESS
WOS记录号	WOS:000279294400016
资助机构	Natural Science Foundation of Shandong Province, China(2006ZRB01973) ; Natural Science Foundation of Shandong Province, China(2006ZRB01973) ; National Natural Science Foundation of China(40821091 ; National Natural Science Foundation of China(40821091 ; 40325011) ; 40325011) ; Natural Science Foundation of Shandong Province, China(2006ZRB01973) ; Natural Science Foundation of Shandong Province, China(2006ZRB01973) ; National Natural Science Foundation of China(40821091 ; National Natural Science Foundation of China(40821091 ; 40325011) ; 40325011) ; Natural Science Foundation of Shandong Province, China(2006ZRB01973) ; Natural Science Foundation of Shandong Province, China(2006ZRB01973) ; National Natural Science Foundation of China(40821091 ; National Natural Science Foundation of China(40821091 ; 40325011) ; 40325011) ; Natural Science Foundation of Shandong Province, China(2006ZRB01973) ; Natural Science Foundation of Shandong Province, China(2006ZRB01973) ; National Natural Science Foundation of China(40821091 ; National Natural Science Foundation of China(40821091 ; 40325011) ; 40325011)
内容类型	期刊论文
源URL	[http://ir.iggcas.ac.cn/handle/132A11/72484]
专题	中国科学院地质与地球物理研究所
通讯作者	Chen Guanjun
作者单位	1.Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China 2.Chinese Acad Sci, Biogeomagnetism Grp, Paleomagnetism & Geochronol Lab SKL LE, Inst Geol & Geophys, Beijing 100029, Peoples R China 3.Shandong Univ, Marine Coll, Weihai 264209, Peoples R China
推荐引用方式 GB/T 7714	Li Jinhua,Ge Xin,Zhang Xiaokui,et al. Recover vigorous cells of Magnetospirillum magneticum AMB-1 by capillary magnetic separation[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2010,28(4):826-831.
APA	Li Jinhua,Ge Xin,Zhang Xiaokui,Chen Guanjun,&Pan Yongxin.(2010).Recover vigorous cells of Magnetospirillum magneticum AMB-1 by capillary magnetic separation.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,28(4),826-831.
MLA	Li Jinhua,et al."Recover vigorous cells of Magnetospirillum magneticum AMB-1 by capillary magnetic separation".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 28.4(2010):826-831.