Gene expression of conditioned locomotion and context-specific locomotor sensitization controlled by morphine-associated environment

	Gene expression of conditioned locomotion and context-specific locomotor sensitization controlled by morphine-associated environment
	Liang, Jing1 ; Chen, Ji-Huan 2; Chen, Xiao-Hong 3; Peng, Yong-Hua 1; Zheng, Xi-Geng1 ; Zheng, XG (reprint author), Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, Beijing 100101, Peoples R China.
刊名	BEHAVIOURAL BRAIN RESEARCH
	2011
卷号	216 期号:1 页码:321-331
关键词	Morphine Conditioned response Sensitization Nucleus accumbens Microarray
ISSN号	0166-4328
产权排序	1
文献子类	Article
英文摘要	The nucleus accumbens (NAc) is involved in contextual drug associations, which might be particularly important for environmental cue-induced relapse to drug seeking. In the present study, rats were first administered repeated morphine for 5 days (5 mg/kg, i.p.) in a contextually paired and unpaired design. After reexposure to the morphine-associated environment, which induced conditioned locomotor activity in the morphine-paired group, we performed a rat 27k 70-mer oligo array to profile gene expression in the NAc. One hundred fifty-five upregulated and 88 downregulated genes were found in the paired group compared with the unpaired group. Eight gene transcripts were then selected to confirm their alterations by quantitative real-time polymerase chain reaction (qRT-PCR). The identified genes generally play important roles in neuroactive receptor-ligand interactions, synapse plasticity, ion transport, and protein phosphorylation. Furthermore, the expression of the eight selected genes that were identified and confirmed to show significant fold changes in the first microarray experiment were again measured with qRT-PCR after morphine challenge (2 mg/kg, i.p.). As expected, 2 mg/kg morphine-induced context-specific sensitization. Meanwhile, mRNA expression of the selected genes showed marked upregulation in the morphine-paired group compared with the unpaired and acute groups. These results suggest that alterations in the expression of the identified genes in the NAc may contribute to the neuroplasticity underlying contextual cue-induced relapse to drug use. (C) 2010 Elsevier B.V. All rights reserved.
学科主题	Cognitive Neuroscience
URL标识	查看原文
资助项目	一室郑希耕课题组
语种	英语
WOS记录号	WOS:000285318500044
资助机构	National Foundation of Natural Science [30470578, 30900400, 30770722] ; Institute of Psychology, Chinese Academy of Sciences [08CX053005]
公开日期	2011-11-11
内容类型	期刊论文
源URL	[http://ir.psych.ac.cn/handle/311026/6918]
专题	心理研究所_健康与遗传心理学研究室
通讯作者	Zheng, XG (reprint author), Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, Beijing 100101, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, Beijing 100101, Peoples R China 2.Scripps Res Inst, Mol & Integrat Neurosci Dept, La Jolla, CA 92037 USA 3.CapitalBio Corp, Beijing 102206, Peoples R China
推荐引用方式 GB/T 7714	Liang, Jing,Chen, Ji-Huan,Chen, Xiao-Hong,et al. Gene expression of conditioned locomotion and context-specific locomotor sensitization controlled by morphine-associated environment[J]. BEHAVIOURAL BRAIN RESEARCH,2011,216(1):321-331.
APA	Liang, Jing,Chen, Ji-Huan,Chen, Xiao-Hong,Peng, Yong-Hua,Zheng, Xi-Geng,&Zheng, XG .(2011).Gene expression of conditioned locomotion and context-specific locomotor sensitization controlled by morphine-associated environment.BEHAVIOURAL BRAIN RESEARCH,216(1),321-331.
MLA	Liang, Jing,et al."Gene expression of conditioned locomotion and context-specific locomotor sensitization controlled by morphine-associated environment".BEHAVIOURAL BRAIN RESEARCH 216.1(2011):321-331.