Microalgae in Microwell Arrays Exhibit Differences with Those in Flasks: Evidence from Growth Rate, Cellular Carotenoid, and Oxygen Production

doi:10.3389/fpls.2077.02257

CORC > 重庆绿色智能技术研究院 > 中国科学院重庆绿色智能技术研究院 > 水质生物转化研究中心

	Microalgae in Microwell Arrays Exhibit Differences with Those in Flasks: Evidence from Growth Rate, Cellular Carotenoid, and Oxygen Production
	Zhang, Ping 1,2; Xiao, Yan2 ; Li, Zhe1,2 ; Guo, Jinsong 1; Lu, Lunhui2
刊名	FRONTIERS IN PLANT SCIENCE
	2018-01-10
卷号	8 页码:10
关键词	microwell arrays physiological response Chlorella vulgaris Raman spectroscopy non-invasive microtest
ISSN号	1664-462X
DOI	10.3389/fpls.2077.02257
英文摘要	Microalgae are cultivated in macro-scale reactors traditionally and the relevant knowledge is based on bulk analysis. Whether the knowledge and laws are true for cells under micro-cultivation is still unknown. To better understand microalgal physiology, micro-cultivation of microalgae, and unicellular tracking and analysis of its response in vivo is necessary. In the study, cellular responses of Chlorella vulgaris to micro-cultivation is studied, with cells in flasks as a control. Five different microwell depths ranging from 10 to 200 mu m with a fixed diameter of 100 mu m, and four diameter levels from 30 to 200 mu m with a fixed depth 60 mu m were investigated. Unicellular dynamics showed that cell number differences among various types of microwells with different initial cell numbers decreased as cultivation processed. Besides, the specific growth rate of C. vulgaris on microwell arrays was much higher than that in flasks and so cells on microwell arrsys can be much sensitive to pollutants. Thus, the interesting characteristics may be used in cell sensor applications to enhance sensitivity. The specific growth rate of C. vulgaris on microwell arrays decreased gradually as the microwell diameter increased from 30 to 2001 mu m while presented a unimodal trend as depth decreased from 200 to 101 mu m. Furthermore, we used Raman Spectroscopy and Non-invasive Micro-test Technique to analyze cellular responses in microwells for the first time to track the changes in vivo. Results indicated that unicellular carotenoid content increased as microwells became larger and shallower. The flow rate of oxygen rose gradually as the depth increased from 10 to 100 mu m, but then decreased rapidly as the depth deepened to 200 mu m. In fact, it is a combined result of cell physiology and density. In summary, cells in microwells with the diameter/depth ratio similar to 1 owned the highest specific growth rates and oxygen flow rates. Simulations also suggested that better mass transfer occurred in microwells with higher diameter-to-depth ratios.
资助项目	National Natural Science Foundation of China[51679226] ; National Natural Science Foundation of China[51779240] ; Chongqing Research Program of Basic Research and Frontier Technology[cstc2015jcyjBX0006] ; "Light of West China" Program - Chinese Academy of Sciences
WOS研究方向	Plant Sciences
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000419691700001
内容类型	期刊论文
源URL	[http://172.16.51.4:88/handle/2HOD01W0/85]
专题	水质生物转化研究中心
通讯作者	Xiao, Yan; Li, Zhe
作者单位	1.Chongqing Univ, Coll Urban Construct & Environm Engn, Dept Environm Sci & Engn, Chongqing, Peoples R China 2.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, CAS Key Lab Reservoir Environm, Chongqing, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Ping,Xiao, Yan,Li, Zhe,et al. Microalgae in Microwell Arrays Exhibit Differences with Those in Flasks: Evidence from Growth Rate, Cellular Carotenoid, and Oxygen Production[J]. FRONTIERS IN PLANT SCIENCE,2018,8:10.
APA	Zhang, Ping,Xiao, Yan,Li, Zhe,Guo, Jinsong,&Lu, Lunhui.(2018).Microalgae in Microwell Arrays Exhibit Differences with Those in Flasks: Evidence from Growth Rate, Cellular Carotenoid, and Oxygen Production.FRONTIERS IN PLANT SCIENCE,8,10.
MLA	Zhang, Ping,et al."Microalgae in Microwell Arrays Exhibit Differences with Those in Flasks: Evidence from Growth Rate, Cellular Carotenoid, and Oxygen Production".FRONTIERS IN PLANT SCIENCE 8(2018):10.